1. Department of Translational Imaging, Houston Methodist Research Institute;
2. Department of Obstetrics and Gynecology, Houston Methodist Hospital, Houston, TX, USA
3. Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medical College, New York, NY, USA
4. Department of Pathology, Chonnam National University Hospital, Gwangju, Korea
A novel cell-permeable compound, CypH-1, that is non-fluorescent at neutral pH, but fluoresces under mildly acidic conditions with a near infrared maximum emission wavelength was designed for the detection of tumors in the clinical setting. The potential of CypH-1 in ovarian cancer imaging was demonstrated using a murine model. The intraperitoneally administered CypH-1 results in a robust fluorescence signal of discrete neoplastic lesions with millimeter range resolution within few hours. Moreover, fluorescence signal is strikingly enhanced at peripheral regions of tumors at the microscopic level suggesting a sharp physiological difference at the tumor/normal tissue interface. This robust acid-activated imaging agent is expected to have significant impact in broad surgical and diagnostic applications.
Keywords: fluorogenic dye, pH indicator, tumor microenvironment, acidic, ovarian cancer.