ISSN: 1838-7640Theranostics
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Theranostics 2023; 13(2):639-658. doi:10.7150/thno.79953 This issue Cite
Research Paper
Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers
1. Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
2. School of Biomedical Engineering, Hainan University, Haikou, Hainan 570228, China
† These authors contributed equally
Citation:
Huang S, Li B, Liu Z, Xu M, Lin D, Hu J, Cao D, Pan Q, Zhang J, Yuan J, Luo Q, Zhang Z. Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics 2023; 13(2):639-658. doi:10.7150/thno.79953. https://www.thno.org/v13p0639.htm
Other stylesAbstract
Rationale: Hepatic lymphatics are essential for liver homeostasis and immune function. However, the 3D structure and spatial distribution of hepatic lymphatic vessels (LVs) need to be confirmed. Moreover, the molecular information of hepatic lymphatic endothelial cells (LyECs) needs to be further studied. The bottleneck is the lack of specific markers or labeling methods for hepatic lymphatic endothelial cells (LyECs)
Methods: Here, we proposed a method for the spatiotemporal sequential injection of antibodies (STSI-Ab) to selectively label hepatic LyECs in vivo. In addition, we also developed an efficient hepatic LyEC sorting method and performed deep transcriptome sequencing on hepatic LyECs.
Results: The STSI-Ab method achieved selective labeling of the mouse hepatic lymphatic network. Three-dimensional fluorescence imaging results of the STSI-Ab mouse liver lobe clearly showed that hepatic LVs entangled with the portal vein but were not present in the central vein. The imaging data inspired a novel hepatic lobule structure model with an added set of LVs in the portal area. Furthermore, deep transcriptome sequencing of isolated hepatic LyECs and Masson's trichrome staining results suggested that hepatic LyECs might be an important source of collagen fibers deposited in the portal area during the process of liver fibrosis and bile duct ligation (BDL).
Conclusions: We proposed an STSI-Ab method for selectively labeling hepatic LVs, distinguishing the hepatic LVs from other vessels, and mapping their 3D structure. This study opens an avenue for understanding hepatic lymphatic structure and it will be very beneficial to the study of hepatic LyEC functions.
Keywords: hepatic lymphatic vessels, in vivo fluorescence labeling and 3D imaging, hepatic lymphatic endothelial cell isolation, transcriptome sequencing, liver disease
Citation styles
APA
Huang, S., Li, B., Liu, Z., Xu, M., Lin, D., Hu, J., Cao, D., Pan, Q., Zhang, J., Yuan, J., Luo, Q., Zhang, Z. (2023). Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics, 13(2), 639-658. https://doi.org/10.7150/thno.79953.
ACS
Huang, S.; Li, B.; Liu, Z.; Xu, M.; Lin, D.; Hu, J.; Cao, D.; Pan, Q.; Zhang, J.; Yuan, J.; Luo, Q.; Zhang, Z. Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics 2023, 13 (2), 639-658. DOI: 10.7150/thno.79953.
NLM
Huang S, Li B, Liu Z, Xu M, Lin D, Hu J, Cao D, Pan Q, Zhang J, Yuan J, Luo Q, Zhang Z. Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics 2023; 13(2):639-658. doi:10.7150/thno.79953. https://www.thno.org/v13p0639.htm
CSE
Huang S, Li B, Liu Z, Xu M, Lin D, Hu J, Cao D, Pan Q, Zhang J, Yuan J, Luo Q, Zhang Z. 2023. Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics. 13(2):639-658.
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