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Theranostics 2017; 7(19):4791-4804. doi:10.7150/thno.20767 This issue Cite

Research Paper

Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles

Tao Su1, 3*, Ya-Bin Wang1*, Dong Han1, 2*, Jing Wang5, Shun Qi4, Lei Gao1, Ya-Hui Shao5, Hong-Yu Qiao2, Jiang-Wei Chen2, Shu-Hui Liang6, Yong-Zhan Nie6, Jia-Yi Li2, Feng Cao1✉

1. Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China;
2. Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China;
3. Department of Cardiology, the 94th Hospital of Chinese PLA, Nanchang 330000, China;
4. Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China;
5. Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China;
6. Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
* These authors contributed equally to this work

✉ Corresponding author: Feng Cao, M.D., Ph.D. FACC, Department of Cardiology, Chinese PLA General Hospital, 28# Fuxing Road, Beijing 100853, China Tel: +86-10-55499138 E-mail: wind8828com or fengcaoedu.cn or fengcao8828com

Citation:
Su T, Wang YB, Han D, Wang J, Qi S, Gao L, Shao YH, Qiao HY, Chen JW, Liang SH, Nie YZ, Li JY, Cao F. Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles. Theranostics 2017; 7(19):4791-4804. doi:10.7150/thno.20767. https://www.thno.org/v07p4791.htm
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Abstract

Graphic abstract

Background and Aims: Angiogenesis is an important pathological process during progression of plaque formation, which can result in plaque hemorrhage and vulnerability. This study aims to explore non-invasive imaging of angiogenesis in atherosclerotic plaque through magnetic resonance imaging (MRI) and positron emission tomography (PET) by using GEBP11 peptide targeted magnetic iron oxide nanoparticles in a rabbit model of atherosclerosis.

Methods: The dual-modality imaging probe was constructed by coupling 2, 3-dimercaptosuccinnic acid-coated paramagnetic nanoparticles (DMSA-MNPs) and the PET 68Ga chelator 1,4,7-triazacyclononane-N, N', N''-triacetic acid (NOTA) to GEBP11 peptide. The atherosclerosis model was induced in New Zealand white rabbits by abdominal aorta balloon de-endothelialization and atherogenic diet for 12 weeks. The plaque areas in abdominal artery were detected by ultrasound imaging and Oil Red O staining. Immunofluorescence staining and Prussian blue staining were applied respectively to investigate the affinity of GEBP11 peptide. MTT and flow cytometric analysis were performed to detect the effects of NGD-MNPs on cell proliferation, cell cycle and apoptosis in Human umbilical vein endothelial cells (HUVECs). In vivo MRI and PET imaging of atherosclerotic plaque were carried out at different time points after intravenous injection of nanoparticles.

Results: The NGD-MNPs with hydrodynamic diameter of 130.8 nm ± 7.1 nm exhibited good imaging properties, high stability, low immunogenicity and little cytotoxicity. In vivo PET/MR imaging revealed that 68Ga-NGD-MNPs were successfully applied to visualize atherosclerotic plaque angiogenesis in the rabbit abdominal aorta. Prussian blue and CD31 immunohistochemical staining confirmed that NGD-MNPs were well co-localized within the blood vessels' plaques.

Conclusion: 68Ga-NGD-MNPs might be a promising MR and PET dual imaging probe for visualizing the vulnerable plaques.

Keywords: Atherosclerosis, GEBP11 peptide, Magnetic nanoparticles, Multimodality imaging.

Citation styles

APA
Su, T., Wang, Y.B., Han, D., Wang, J., Qi, S., Gao, L., Shao, Y.H., Qiao, H.Y., Chen, J.W., Liang, S.H., Nie, Y.Z., Li, J.Y., Cao, F. (2017). Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles. Theranostics, 7(19), 4791-4804. https://doi.org/10.7150/thno.20767.

ACS
Su, T.; Wang, Y.B.; Han, D.; Wang, J.; Qi, S.; Gao, L.; Shao, Y.H.; Qiao, H.Y.; Chen, J.W.; Liang, S.H.; Nie, Y.Z.; Li, J.Y.; Cao, F. Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles. Theranostics 2017, 7 (19), 4791-4804. DOI: 10.7150/thno.20767.

NLM
Su T, Wang YB, Han D, Wang J, Qi S, Gao L, Shao YH, Qiao HY, Chen JW, Liang SH, Nie YZ, Li JY, Cao F. Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles. Theranostics 2017; 7(19):4791-4804. doi:10.7150/thno.20767. https://www.thno.org/v07p4791.htm

CSE
Su T, Wang YB, Han D, Wang J, Qi S, Gao L, Shao YH, Qiao HY, Chen JW, Liang SH, Nie YZ, Li JY, Cao F. 2017. Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles. Theranostics. 7(19):4791-4804.

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