Theranostics 2017; 7(13):3326-3337. doi:10.7150/thno.17089 This issue
1. Krasnoyarsk State Medical University named after Professor V. F. Voyno-Yasenetsky, Krasnoyarsk, Russia;
2. Federal Research Center, KSC Siberian branch of Russian Academy of Science, Krasnoyarsk, Russia;
3. Siberian Federal University, Krasnoyarsk, Russia;
4. University of Ottawa, Department of Chemistry and Biomolecular Sciences, Ottawa, Ontario Canada;
5. Institute of Computational Modeling RAS SB, Krasnoyarsk, Russia.
Biomedical applications of magnetic nanoparticles under the influence of a magnetic field have been proved useful beyond expectations in cancer therapy. Magnetic nanoparticles are effective heat mediators, drug nanocarriers, and contrast agents; various strategies have been suggested to selectively target tumor cancer cells. Our study presents magnetodynamic nanotherapy using DNA aptamer-functionalized 50 nm gold-coated magnetic nanoparticles exposed to a low frequency alternating magnetic field for selective elimination of tumor cells in vivo. The cell specific DNA aptamer AS-14 binds to the fibronectin protein in Ehrlich carcinoma hence helps deliver the gold-coated magnetic nanoparticles to the mouse tumor. Applying an alternating magnetic field of 50 Hz at the tumor site causes the nanoparticles to oscillate and pull the fibronectin proteins and integrins to the surface of the cell membrane. This results in apoptosis followed by necrosis of tumor cells without heating the tumor, adjacent healthy cells and tissues. The aptamer-guided nanoparticles and the low frequency alternating magnetic field demonstrates a unique non-invasive nanoscalpel technology for precise cancer surgery at the single cell level.
Keywords: cancer therapy, gold coated magnetic nanoparticles, DNA aptamers, low frequency alternating magnetic field, fibronectin, integrin, apoptosis, necrosis.