Theranostics 2022; 12(15):6779-6808. doi:10.7150/thno.74308 This issue

Review

Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis

Shijun Wang1#, Shu Zhu1#, Ziqi Kang1#, Yidan Chen2, Xiancheng Liu2, Zixin Deng1, Kun Hu1, Guixue Wang2,3✉, Yuchan Zhang1✉, Guangchao Zang1,3✉

1. Institute of Life Science, and Laboratory of Tissue and Cell Biology, Lab Teaching & Management Center, Chongqing Medical University, Chongqing, 400016, China.
2. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China.
3. Jinfeng Laboratory, Chongqing, 401329, China.
#These authors contributed equally to this work.

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Citation:
Wang S, Zhu S, Kang Z, Chen Y, Liu X, Deng Z, Hu K, Wang G, Zhang Y, Zang G. Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis. Theranostics 2022; 12(15):6779-6808. doi:10.7150/thno.74308. Available from https://www.thno.org/v12p6779.htm

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Abstract

Graphic abstract

The potential-resolved strategy has gradually demonstrated its distinct values in electrochemiluminescence (ECL) bio-sensing due to its superior characteristics, such as low instrument requirement, short assay time, and improved sample throughput, in conjunction with spatial- and spectrum-resolved techniques. It has recently been widely generalized into versatile multiple-signal ECL analytic platforms, especially in ratiometric and multiplex ECL sensors, in accordance with some specific principles. Furthermore, luminophore pairs with potential- and wavelength-resolved properties have been utilized to visualize biosensors that display multiple colors depending on analyte concentration. However, only a few comprehensive reports on the principles, construction, and application of various ECL sensors in potential-resolved schemes have been published. This review aims to recount the potential-resolved strategy applying to (a) ratiometric ECL sensors, (b) multiplex ECL sensors, and (c) multicolor ECL sensors and to discuss the distinctions and connections among the application principles of these strategies. Finally, the future prospects of ECL-based potential-resolved analysis are explored.

Keywords: electrochemiluminescence, potential-resolved strategy, ratiometric electrochemiluminescence sensors, multiplex electrochemiluminescence sensors, multicolor electrochemiluminescence sensors