Theranostics 2019; 9(20):5784-5796. doi:10.7150/thno.36545 This issue Cite

Research Paper

Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging

Zhongyuan Chen1, Yueting Peng1, Xiaoxue Xie1, Yi Feng1, Tingting Li1,3, Shun Li1,3, Xiang Qin1,3, Hong Yang1,3, Chunhui Wu1,3, Chuan Zheng2, Jie Zhu2, Fengming You2, Yiyao Liu1,2,3✉

1. Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, P.R. China
2. Hospital of Chengdu University of Traditional Chinese Medicine, No. 39 Shi-er-qiao Road, Chengdu 610072, Sichuan, P.R. China
3. Center for Information in Biology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, P.R. China

Citation:
Chen Z, Peng Y, Xie X, Feng Y, Li T, Li S, Qin X, Yang H, Wu C, Zheng C, Zhu J, You F, Liu Y. Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging. Theranostics 2019; 9(20):5784-5796. doi:10.7150/thno.36545. https://www.thno.org/v09p5784.htm
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Abstract

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Background & Aims: The use of antisense oligonucleotide-based nanosystems for the detection and regulation of tumor-related gene expression is thought to be a promising approach for cancer diagnostics and therapies. Herein, we report that a cubic-shaped iron oxide nanoparticle (IONC) core nanobeacon is capable of delivering an HSP90α mRNA-specific molecular beacon (HSP90-MB) into living cells and enhancing T2-weighted MR imaging in a tumor model.

Methods: The nanobeacons were built with IONC, generation 4 poly(amidoamine) dendrimer (G4 PAMAM), Pluronic P123 (P123) and HSP90-MB labeled with a quencher (BHQ1) and a fluorophore (Alexa Fluor 488).

Results: After internalization by malignant cells overexpressing HSP90α, the fluorescence of the nanobeacon was recovered, thus distinguishing cancer cells from normal cells. Meanwhile, MB-mRNA hybridization led to enzyme activity that degraded DNA/RNA hybrids and resulted in downregulation of HSP90α at both the mRNA and protein levels. Furthermore, the T2-weighted MR imaging ability of the nanobeacons was increased after PAMAM and P123 modification, which exhibited good biocompatibility and hemocompatibility.

Conclusions: The nanobeacons show promise for applicability to tumor-related mRNA detection, regulation and multiscale imaging in the fields of cancer diagnostics and therapeutics.

Keywords: iron oxide nanocubes, poly(amidoamine) dendrimer, cancer theranostics, HSP90α, molecular beacon


Citation styles

APA
Chen, Z., Peng, Y., Xie, X., Feng, Y., Li, T., Li, S., Qin, X., Yang, H., Wu, C., Zheng, C., Zhu, J., You, F., Liu, Y. (2019). Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging. Theranostics, 9(20), 5784-5796. https://doi.org/10.7150/thno.36545.

ACS
Chen, Z.; Peng, Y.; Xie, X.; Feng, Y.; Li, T.; Li, S.; Qin, X.; Yang, H.; Wu, C.; Zheng, C.; Zhu, J.; You, F.; Liu, Y. Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging. Theranostics 2019, 9 (20), 5784-5796. DOI: 10.7150/thno.36545.

NLM
Chen Z, Peng Y, Xie X, Feng Y, Li T, Li S, Qin X, Yang H, Wu C, Zheng C, Zhu J, You F, Liu Y. Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging. Theranostics 2019; 9(20):5784-5796. doi:10.7150/thno.36545. https://www.thno.org/v09p5784.htm

CSE
Chen Z, Peng Y, Xie X, Feng Y, Li T, Li S, Qin X, Yang H, Wu C, Zheng C, Zhu J, You F, Liu Y. 2019. Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging. Theranostics. 9(20):5784-5796.

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