The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation
Brian P. Danysh1,*, Pamela E. Constantinou1,*, Ekaterina Y. Lukianova-Hleb1,*, Dmitri O. Lapotko1,2, Daniel D. Carson1,3,✉
1. Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA; 2. Department of Physics and Astronomy, Rice University, Houston, TX 77005, USA; 3. Department of Biochemistry and Molecular Biology, MD Anderson Cancer Center, Houston, TX 77030, USA. * Contributed equally to this work.
✉ Corresponding author: dcarsonedu
Danysh BP, Constantinou PE, Lukianova-Hleb EY, Lapotko DO, Carson DD. The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation. Theranostics 2012; 2(8):777-787. doi:10.7150/thno.4494. https://www.thno.org/v02p0777.htm
MUC1 is a large, heavily glycosylated transmembrane glycoprotein that is proposed to create a protective microenvironment in many adenocarcinomas. Here we compare MUC1 and the well studied cell surface receptor target, EGFR, as gold nanoparticle (AuNP) targets and their subsequent vapor nanobubble generation efficacy in the human epithelial cell line, HES. Although EGFR and MUC1 were both highly expressed in these cells, TEM and confocal images revealed MUC1 as a superior target for nanoparticle intracellular accumulation and clustering. The MUC1-targeted AuNP intracellular clusters also generated significantly larger vapor nanobubbles. Our results demonstrate the promising opportunities MUC1 offers to improve the efficacy of targeted nanoparticle based approaches.