Full Text | PDF

Theranostics 2024; 14(2):510-527. doi:10.7150/thno.89008 This issue Cite

Research Paper

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Yi Sun^1,3,4,5,#, Quanbo Liu^1,3,4,5,#, Yiming Qin^1,3,4,5, Yan Xu^2,3,4,5, Jinyun Zhao^1,3,4,5, Yong Xie^1,3,4,5, Chengjun Li^1,3,4,5, Tian Qin^1,3,4,5, Yuxin Jin^1,3,4,5, Liyuan Jiang^1,3,4,5, Yong Cao^1,3,4,5, Hongbin Lu^2,3,4,5,✉, Jianzhong Hu^1,3,4,5,✉

1. Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China.
2. Department of Sports Medicine, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China.
3. Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Xiangya Road 87, Changsha 410008, China.
4. Hunan Engineering Research Center of Sports and Health, Changsha 410008, China.
5. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China.
^#These authors contributed equally.

✉ Corresponding authors: Jianzhong Hu, Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China. E-mail: jianzhonghuedu.cn, 086-13875855748. Hongbin Lu, Department of Sports Medicine, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China. E-mail: hongbinluedu.cn, 086-15874889777. More

Abstract

Rationale: Spinal cord injury (SCI) results in neural tissue damage. However, the limited regenerative capacity of adult mammals' axons upon SCI leads to persistent neurological dysfunction. Thus, exploring the pathways that can enhance axon regeneration in injured spinal cord is of great significance.

Methods: Through the utilization of single-cell RNA sequencing in this research, a distinct subpopulation of bone marrow mesenchymal stem cells (BMSCs) that exhibits the capacity to facilitate axon regeneration has been discovered. Subsequently, the CD271⁺CD56⁺ BMSCs subpopulation was isolated using flow cytometry, and the exosomes derived from this subpopulation (CD271⁺CD56⁺ BMSC-Exos) were extracted and incorporated into a hydrogel to create a sustained release system. The aim was to investigate the therapeutic effects of CD271⁺CD56⁺ BMSC-Exos and elucidate the underlying mechanisms involved in promoting axon regeneration and neural function recovery.

Results: The findings indicate that CD271⁺CD56⁺ BMSC-Exos share similar physical and chemical properties with conventional exosomes. Importantly, in an SCI model, in situ implantation of CD271⁺CD56⁺ BMSC-Exos hydrogel resulted in increased expression of NF and synaptophysin, markers associated with axon regeneration and synapse formation, respectively. This intervention also contributed to improved neural function recovery. In vitro experiments demonstrated that CD271⁺CD56⁺ BMSC-Exos treatment significantly enhanced axon extension distance and increased the number of branches in dorsal root ganglion axons. Moreover, further investigation into the molecular mechanisms underlying CD271⁺CD56⁺ BMSC-Exos-mediated axon regeneration revealed the crucial involvement of the miR-431-3p/RGMA axis.

Conclusion: In summary, the implantation of CD271⁺CD56⁺ BMSC-Exos hydrogel presents a promising and effective therapeutic approach for SCI.

Keywords: Bone Marrow Mesenchymal Stem Cell, Single-Cell RNA Sequencing, Spinal cord injury, Axon Regeneration, Exosome

Citation styles

©2024 Ivyspring International Publisher. Terms of use