Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2023; 13(9):3041-3063. doi:10.7150/thno.80901 This issue Cite

Review

Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens

Sana Khan Khilji1,2, Charlotte Op 't Hoog1,3, David Warschkau1,2, Jost Lühle1,2, Felix Goerdeler1,2, Anika Freitag1,4, Peter H. Seeberger1,2, Oren Moscovitz1✉

1. Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
2. Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
3. Graduate School of Life Sciences, Utrecht University, 3584 CH Utrecht, Netherlands.
4. Institute of Chemistry, University of Potsdam, 14476 Potsdam, Germany.

✉ Corresponding author: oren.moscovitzmpg.de.

Citation:
Khilji SK, Op 't Hoog C, Warschkau D, Lühle J, Goerdeler F, Freitag A, Seeberger PH, Moscovitz O. Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens. Theranostics 2023; 13(9):3041-3063. doi:10.7150/thno.80901. https://www.thno.org/v13p3041.htm
Other styles

File import instruction

Abstract

Graphic abstract

Attached to proteins, lipids, or forming long, complex chains, glycans represent the most versatile post-translational modification in nature and surround all human cells. Unique glycan structures are monitored by the immune system and differentiate self from non-self and healthy from malignant cells. Aberrant glycosylations, termed tumour-associated carbohydrate antigens (TACAs), are a hallmark of cancer and are correlated with all aspects of cancer biology. Therefore, TACAs represent attractive targets for monoclonal antibodies for cancer diagnosis and therapy. However, due to the thick and dense glycocalyx as well as the tumour micro-environment, conventional antibodies often suffer from restricted access and limited effectiveness in vivo. To overcome this issue, many small antibody fragments have come forth, showing similar affinity with better efficiency than their full-length counterparts. Here we review small antibody fragments against specific glycans on tumour cells and highlight their advantages over conventional antibodies.

Keywords: fragments, TACAs, cancer, aberrant glycosylation, CAR T-cell, ScFv, nanobody, carbohydrates, tumor-associated carbohydrate antigens, chimeric antigen receptors, single-chain variable fragment, single-chain fragment variable, tumor, Tumour, single domain antibodies, cancer antigens, glycans, immunology, cancer glycans

Citation styles

APA
Khilji, S.K., Op 't Hoog, C., Warschkau, D., Lühle, J., Goerdeler, F., Freitag, A., Seeberger, P.H., Moscovitz, O. (2023). Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens. Theranostics, 13(9), 3041-3063. https://doi.org/10.7150/thno.80901.

ACS
Khilji, S.K.; Op 't Hoog, C.; Warschkau, D.; Lühle, J.; Goerdeler, F.; Freitag, A.; Seeberger, P.H.; Moscovitz, O. Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens. Theranostics 2023, 13 (9), 3041-3063. DOI: 10.7150/thno.80901.

NLM
Khilji SK, Op 't Hoog C, Warschkau D, Lühle J, Goerdeler F, Freitag A, Seeberger PH, Moscovitz O. Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens. Theranostics 2023; 13(9):3041-3063. doi:10.7150/thno.80901. https://www.thno.org/v13p3041.htm

CSE
Khilji SK, Op 't Hoog C, Warschkau D, Lühle J, Goerdeler F, Freitag A, Seeberger PH, Moscovitz O. 2023. Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens. Theranostics. 13(9):3041-3063.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use