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Theranostics 2023; 13(3):1150-1164. doi:10.7150/thno.75871 This issue Cite

Research Paper

Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair

Christian Biederbick1#, Jan C. Heinemann1,2#, Sarah Rieck1, Florian Winkler1, Annika Ottersbach1,2, Miriam Schiffer1,2, Georg D. Duerr2,3, Dietmar Eberbeck4, Michael Hesse1, Wilhelm Röll2, Daniela Wenzel5,1✉

1. Institute of Physiology I, Life&Brain Center, Medical Faculty, University of Bonn, Bonn, Germany
2. Department of Cardiac Surgery, Medical Faculty, University of Bonn, Bonn, Germany
3. Department of Cardiovascular Surgery, University Medical Center Mainz, Johannes Gutenberg University, Mainz, Germany
4. Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany
5. Institute of Physiology, Department of Systems Physiology, Medical Faculty, Ruhr University of Bochum, Bochum, Germany
# Contributed equally

✉ Corresponding author: Daniela Wenzel, MD. Institute of Physiology, Department of Systems Physiology, Medical Faculty, Ruhr University of Bochum, Universitätsstr. 150, 44801 Bochum, Germany. Tel: 0049/234/32-29100 Email: daniela.wenzelde

Citation:
Biederbick C, Heinemann JC, Rieck S, Winkler F, Ottersbach A, Schiffer M, Duerr GD, Eberbeck D, Hesse M, Röll W, Wenzel D. Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair. Theranostics 2023; 13(3):1150-1164. doi:10.7150/thno.75871. https://www.thno.org/v13p1150.htm
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Abstract

Graphic abstract

Background: The regenerative potential of the heart after injury is limited. Therefore, cell replacement strategies have been developed. However, the engraftment of transplanted cells in the myocardium is very inefficient. In addition, the use of heterogeneous cell populations precludes the reproducibility of the outcome.

Methods: To address both issues, in this proof of principle study, we applied magnetic microbeads for combined isolation of eGFP+ embryonic cardiac endothelial cells (CECs) by antigen-specific magnet-associated cell sorting (MACS) and improved engraftment of these cells in myocardial infarction by magnetic fields.

Results: MACS provided CECs of high purity decorated with magnetic microbeads. In vitro experiments revealed that the angiogenic potential of microbead-labeled CECs was preserved and the magnetic moment of the cells was strong enough for site-specific positioning by a magnetic field. After myocardial infarction in mice, intramyocardial CEC injection in the presence of a magnet resulted in a strong improvement of cell engraftment and eGFP+ vascular network formation in the hearts. Hemodynamic and morphometric analysis demonstrated augmented heart function and reduced infarct size only when a magnetic field was applied.

Conclusion: Thus, the combined use of magnetic microbeads for cell isolation and enhanced cell engraftment in the presence of a magnetic field is a powerful approach to improve cell transplantation strategies in the heart.

Keywords: magnetic microbeads, cell therapy, cell engraftment, myocardial repair, endothelial cells

Citation styles

APA
Biederbick, C., Heinemann, J.C., Rieck, S., Winkler, F., Ottersbach, A., Schiffer, M., Duerr, G.D., Eberbeck, D., Hesse, M., Röll, W., Wenzel, D. (2023). Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair. Theranostics, 13(3), 1150-1164. https://doi.org/10.7150/thno.75871.

ACS
Biederbick, C.; Heinemann, J.C.; Rieck, S.; Winkler, F.; Ottersbach, A.; Schiffer, M.; Duerr, G.D.; Eberbeck, D.; Hesse, M.; Röll, W.; Wenzel, D. Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair. Theranostics 2023, 13 (3), 1150-1164. DOI: 10.7150/thno.75871.

NLM
Biederbick C, Heinemann JC, Rieck S, Winkler F, Ottersbach A, Schiffer M, Duerr GD, Eberbeck D, Hesse M, Röll W, Wenzel D. Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair. Theranostics 2023; 13(3):1150-1164. doi:10.7150/thno.75871. https://www.thno.org/v13p1150.htm

CSE
Biederbick C, Heinemann JC, Rieck S, Winkler F, Ottersbach A, Schiffer M, Duerr GD, Eberbeck D, Hesse M, Röll W, Wenzel D. 2023. Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair. Theranostics. 13(3):1150-1164.

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