Theranostics 2022; 12(3):1161-1172. doi:10.7150/thno.67515 This issue Cite

Research Paper

Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration

Francis M. Chen1✉, Joyce KY Tse1, Leigang Jin2,3, Chui Yiu Bamboo Chook1, Fung Ping Leung1, Gary Tse4,5, Connie W. Woo2,3, Aimin Xu2,3,6, Ajay Chawla7, Xiao Yu Tian8, Ting-Fung Chan1,9, Wing Tak Wong1,9✉

1. School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Hong Kong, China.
2. State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, Hong Kong, China.
3. Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong, China.
4. Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China.
5. Kent and Medway Medical School, Canterbury, United Kingdom.
6. Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
7. Merck Research Labs, SAF-803, South San Francisco, CA, USA.
8. School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.
9. State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.

Citation:
Chen FM, Tse JKY, Jin L, Chook CYB, Leung FP, Tse G, Woo CW, Xu A, Chawla A, Tian XY, Chan TF, Wong WT. Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration. Theranostics 2022; 12(3):1161-1172. doi:10.7150/thno.67515. https://www.thno.org/v12p1161.htm
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Abstract

Graphic abstract

Aims: Neonatal immunity is functionally immature and skewed towards a TH2-driven, anti-inflammatory profile. This neonatal immunotolerance is partly driven by the type 2 cytokines: interleukin-4 (IL-4) and interleukin-13 (IL-13). Studies on neonatal cardiac regeneration reveal the beneficial role of an anti-inflammatory response in restoring cardiac function after injury. However, the role of an imbalanced immune repertoire observed in neonates on tissue regeneration is poorly understood; specifically, whether IL-4 and IL-13 actively modulate neonatal immunity during cardiac injury.

Methods and results: Neonatal mice lacking IL-4 and IL-13 (DKOs) examined at 2 days after birth exhibited reduced anti-inflammatory immune populations with basal cardiac immune populations like adult mice. Examination of neonates lacking IL-4 and IL-13 at 2 days post cardiac ischemic injury, induced on the second day after birth, showed impaired cardiac function compared to their control counterparts. Treatment with either IL-4 or IL-13 cytokine during injury restored both cardiac function and immune population profiles in knockout mice. Examination of IL-4/IL-13 downstream pathways revealed the role of STAT6 in mediating the regenerative response in neonatal hearts. As IL-4/IL-13 drives polarization of alternatively activated macrophages, we also examined the role of IL-4/IL-13 signaling within the myeloid compartment during neonatal cardiac regeneration. Injury of IL-4Rα myeloid specific knockout neonates 2 days after birth revealed that loss of IL-4/IL-13 signaling in macrophages alone was sufficient to impair cardiac regeneration.

Conclusions: Our results confirm that the TH2 cytokines: IL-4 and IL-13, which skews neonatal immunity to a TH2 profile, are necessary for maintaining and mediating an anti-inflammatory response in the neonatal heart, in part through the activation of alternatively activated macrophages, thereby permitting a niche conducive for regeneration.

Keywords: IL-4, IL-13, neonatal heart regeneration, TH2 immunity, alternatively activated macrophages, left anterior descending coronary artery ligation


Citation styles

APA
Chen, F.M., Tse, J.KY., Jin, L., Chook, C.Y.B., Leung, F.P., Tse, G., Woo, C.W., Xu, A., Chawla, A., Tian, X.Y., Chan, T.F., Wong, W.T. (2022). Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration. Theranostics, 12(3), 1161-1172. https://doi.org/10.7150/thno.67515.

ACS
Chen, F.M.; Tse, J.KY.; Jin, L.; Chook, C.Y.B.; Leung, F.P.; Tse, G.; Woo, C.W.; Xu, A.; Chawla, A.; Tian, X.Y.; Chan, T.F.; Wong, W.T. Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration. Theranostics 2022, 12 (3), 1161-1172. DOI: 10.7150/thno.67515.

NLM
Chen FM, Tse JKY, Jin L, Chook CYB, Leung FP, Tse G, Woo CW, Xu A, Chawla A, Tian XY, Chan TF, Wong WT. Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration. Theranostics 2022; 12(3):1161-1172. doi:10.7150/thno.67515. https://www.thno.org/v12p1161.htm

CSE
Chen FM, Tse JKY, Jin L, Chook CYB, Leung FP, Tse G, Woo CW, Xu A, Chawla A, Tian XY, Chan TF, Wong WT. 2022. Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration. Theranostics. 12(3):1161-1172.

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