13.3
Impact Factor
23.90
CiteScore
< 5 days
First Decision
Theranostics 2021; 11(16):7813-7828. doi:10.7150/thno.56173 This issue Cite
Research Paper
1. Helmholtz Zentrum München (GmbH), Neuherberg, Germany, Institute of Biological and Medical Imaging.
2. Technical University of Munich, Germany, School of Medicine, Center for Translational Cancer Research (TranslaTUM), Chair of Biological Imaging.
Non-invasive monitoring of hemodynamic tumor responses to chemotherapy could provide unique insights into the development of therapeutic resistance and inform therapeutic decision-making in the clinic.
Methods: Here, we examined the longitudinal and dynamic effects of the common chemotherapeutic drug Taxotere on breast tumor (KPL-4) blood volume and oxygen saturation using eigenspectra multispectral optoacoustic tomography (eMSOT) imaging over a period of 41 days. Tumor vascular function was assessed by dynamic oxygen-enhanced eMSOT (OE-eMSOT). The obtained in vivo optoacoustic data were thoroughly validated by ex vivo cryoimaging and immunohistochemical staining against markers of vascularity and hypoxia.
Results: We provide the first preclinical evidence that prolonged treatment with Taxotere causes a significant drop in mean whole tumor oxygenation. Furthermore, application of OE-eMSOT showed a diminished vascular response in Taxotere-treated tumors and revealed the presence of static blood pools, indicating increased vascular permeability.
Conclusion: Our work has important translational implications and supports the feasibility of eMSOT imaging for non-invasive assessment of tumor microenvironmental responses to chemotherapy.
Keywords: optoacoustic, photoacoustic, breast cancer, docetaxel, Taxotere, chemotherapy