Theranostics 2023; 13(8):2710-2720. doi:10.7150/thno.82620 This issue Cite

Research Paper

MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects

Vidyani Suryadevara1,#,✉, Mohammad Javad Hajipour1,#, Lisa C. Adams1,#, Nour Mary Aissaoui1,#, Ali Rashidi1, Louise Kiru1, Ashok J. Theruvath1, Ching‐Hsin Huang1, Masahiro Maruyama2, Masanori Tsubosaka2, Jennifer K. Lyons3, Wei (Emma) Wu1, Raheleh Roudi1, Stuart B. Goodman2, Heike E. Daldrup‐Link1

1. Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
2. Department of Orthopedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
3. Department of Veterinary Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, USA.
#These authors contributed equally.

Citation:
Suryadevara V, Hajipour MJ, Adams LC, Aissaoui NM, Rashidi A, Kiru L, Theruvath AJ, Huang C, Maruyama M, Tsubosaka M, Lyons JK, Wu WE, Roudi R, Goodman SB, Daldrup‐Link HE. MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects. Theranostics 2023; 13(8):2710-2720. doi:10.7150/thno.82620. https://www.thno.org/v13p2710.htm
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Abstract

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Rationale: Efficient labeling methods for mesenchymal stem cells (MSCs) are crucial for tracking and understanding their behavior in regenerative medicine applications, particularly in cartilage defects. MegaPro nanoparticles have emerged as a potential alternative to ferumoxytol nanoparticles for this purpose.

Methods: In this study, we employed mechanoporation to develop an efficient labeling method for MSCs using MegaPro nanoparticles and compared their effectiveness with ferumoxytol nanoparticles in tracking MSCs and chondrogenic pellets. Pig MSCs were labeled with both nanoparticles using a custom-made microfluidic device, and their characteristics were analyzed using various imaging and spectroscopy techniques. The viability and differentiation capacity of labeled MSCs were also assessed. Labeled MSCs and chondrogenic pellets were implanted into pig knee joints and monitored using MRI and histological analysis.

Results: MegaPro-labeled MSCs demonstrated shorter T2 relaxation times, higher iron content, and greater nanoparticle uptake compared to ferumoxytol-labeled MSCs, without significantly affecting their viability and differentiation capacity. Post-implantation, MegaPro-labeled MSCs and chondrogenic pellets displayed a strong hypointense signal on MRI with considerably shorter T2* relaxation times compared to adjacent cartilage. The hypointense signal of both MegaPro- and ferumoxytol-labeled chondrogenic pellets decreased over time. Histological evaluations showed regenerated defect areas and proteoglycan formation with no significant differences between the labeled groups.

Conclusion: Our study demonstrates that mechanoporation with MegaPro nanoparticles enables efficient MSC labeling without affecting viability or differentiation. MegaPro-labeled cells show enhanced MRI tracking compared to ferumoxytol-labeled cells, emphasizing their potential in clinical stem cell therapies for cartilage defects.

Keywords: Mesenchymal stem cells (MSCs), MegaPro nanoparticles, mechanoporation, MRI tracking, cartilage defects


Citation styles

APA
Suryadevara, V., Hajipour, M.J., Adams, L.C., Aissaoui, N.M., Rashidi, A., Kiru, L., Theruvath, A.J., Huang, C., Maruyama, M., Tsubosaka, M., Lyons, J.K., Wu, W.E., Roudi, R., Goodman, S.B., Daldrup‐Link, H.E. (2023). MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects. Theranostics, 13(8), 2710-2720. https://doi.org/10.7150/thno.82620.

ACS
Suryadevara, V.; Hajipour, M.J.; Adams, L.C.; Aissaoui, N.M.; Rashidi, A.; Kiru, L.; Theruvath, A.J.; Huang, C.; Maruyama, M.; Tsubosaka, M.; Lyons, J.K.; Wu, W.E.; Roudi, R.; Goodman, S.B.; Daldrup‐Link, H.E. MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects. Theranostics 2023, 13 (8), 2710-2720. DOI: 10.7150/thno.82620.

NLM
Suryadevara V, Hajipour MJ, Adams LC, Aissaoui NM, Rashidi A, Kiru L, Theruvath AJ, Huang C, Maruyama M, Tsubosaka M, Lyons JK, Wu WE, Roudi R, Goodman SB, Daldrup‐Link HE. MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects. Theranostics 2023; 13(8):2710-2720. doi:10.7150/thno.82620. https://www.thno.org/v13p2710.htm

CSE
Suryadevara V, Hajipour MJ, Adams LC, Aissaoui NM, Rashidi A, Kiru L, Theruvath AJ, Huang C, Maruyama M, Tsubosaka M, Lyons JK, Wu WE, Roudi R, Goodman SB, Daldrup‐Link HE. 2023. MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects. Theranostics. 13(8):2710-2720.

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