Theranostics 2022; 12(4):1829-1854. doi:10.7150/thno.68804 This issue Cite

Review

An update of label-free protein target identification methods for natural active products

Zhao Cui1, Caifeng Li2, Peng Chen2,3✉, Hongjun Yang2✉

1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Robot Intelligent Laboratory of Traditional Chinese Medicine, Experimental Research Center, China Academy of Chinese Medical Sciences & MEGAROBO, Beijing, China.

Citation:
Cui Z, Li C, Chen P, Yang H. An update of label-free protein target identification methods for natural active products. Theranostics 2022; 12(4):1829-1854. doi:10.7150/thno.68804. https://www.thno.org/v12p1829.htm
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Abstract

Graphic abstract

Natural active products (NAPs) are derived from chemical substances found in nature that have biological activity and medicinal potential. Screening and revealing the protein targets of NAPs is an indispensable link in the pharmacological and toxicological understanding of NAPs. Proteins are the main factors executing cell functions, and cells rely on the function of proteins to complete various activities in the life cycle. The important mechanism of action of drugs is to regulate cell biological activities by interacting with proteins and other macromolecules. At present, the classic way to screen protein targets is based on the molecular label tracing method, which has a long cycle and changes the molecular structure and pharmacological effects of NAPs. Due to the shortcomings of molecular labelling methods, in recent years, scientists have tried to develop a variety of label-free protein target identification methods for NAPs and have made a certain amount of progress. This article reviews the current protein target identification methods for NAPs with the aim of providing a reference for research on NAP protein targets.

Keywords: protein target identification, protein stability, label-free methods, natural active products, drug discovery


Citation styles

APA
Cui, Z., Li, C., Chen, P., Yang, H. (2022). An update of label-free protein target identification methods for natural active products. Theranostics, 12(4), 1829-1854. https://doi.org/10.7150/thno.68804.

ACS
Cui, Z.; Li, C.; Chen, P.; Yang, H. An update of label-free protein target identification methods for natural active products. Theranostics 2022, 12 (4), 1829-1854. DOI: 10.7150/thno.68804.

NLM
Cui Z, Li C, Chen P, Yang H. An update of label-free protein target identification methods for natural active products. Theranostics 2022; 12(4):1829-1854. doi:10.7150/thno.68804. https://www.thno.org/v12p1829.htm

CSE
Cui Z, Li C, Chen P, Yang H. 2022. An update of label-free protein target identification methods for natural active products. Theranostics. 12(4):1829-1854.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
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