Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2020; 10(22):10092-10105. doi:10.7150/thno.44865 This issue Cite

Research Paper

Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes

Haohan Wu1,2*, Yanni He1,2*, Hao Wu2, Meijun Zhou2, Zhili Xu2, Ran Xiong2, Fei Yan2,3✉, Hongmei Liu1,2✉

1. The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.
2. Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China.
3. CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
*These authors equally contributed to this work.

✉ Corresponding authors: Prof. Hongmei Liu, Tel./Fax 86-19924270557 and E-mail lmeihongcom; Prof. Fei Yan: Tel./Fax: 86-755-86392284 and E-mail fei.yanac.cn.

Citation:
Wu H, He Y, Wu H, Zhou M, Xu Z, Xiong R, Yan F, Liu H. Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes. Theranostics 2020; 10(22):10092-10105. doi:10.7150/thno.44865. https://www.thno.org/v10p10092.htm
Other styles

File import instruction

Abstract

Graphic abstract

Rheumatoid arthritis (RA), a common inflammatory disorder of the joints characterized by synovitis and pannus formation, often results in irreversible joint erosion and disability. Methotrexate (MTX) is the first-line drug against RA, but the therapeutic effects are sub-optimal due to its poor retention at the target sites and systemic side effects. Multifunctional nanoparticles are highly promising agents for minimally invasive, traceable and effective targeted therapy.

Methods: This study developed iRGD peptide-functionalized echogenic liposomes (iELPs) which encapsulates MTX and indocyanine green (ICG) fluorescent probe through the thin film-hydration method.

Results: The resulting iELPs showed high affinity for endothelial cells overexpressing αvβ3 integrin, favorable acoustic response and fluorescence tracking properties. Also, near-infrared (NIR) fluorescence imaging of iELPs and ultrasound-triggered drug release of MTX were proved in a mouse RA model, greatly improving the therapeutic efficacy and reducing MTX side effects. Histological assessment of the articular tissues further revealed significantly lower inflammatory cell infiltration and angiogenesis in the iELPs-treated and sonicated mice.

Conclusion: Our study provides a promising nanoplatform for integrating ultrasound-controlled drug release and NIR fluorescence imaging for RA treatment.

Keywords: Echogenic liposomes, Rheumatoid arthritis, Ultrasound-controlled drug release, Near-infrared fluorescence imaging, iRGD

Citation styles

APA
Wu, H., He, Y., Wu, H., Zhou, M., Xu, Z., Xiong, R., Yan, F., Liu, H. (2020). Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes. Theranostics, 10(22), 10092-10105. https://doi.org/10.7150/thno.44865.

ACS
Wu, H.; He, Y.; Wu, H.; Zhou, M.; Xu, Z.; Xiong, R.; Yan, F.; Liu, H. Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes. Theranostics 2020, 10 (22), 10092-10105. DOI: 10.7150/thno.44865.

NLM
Wu H, He Y, Wu H, Zhou M, Xu Z, Xiong R, Yan F, Liu H. Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes. Theranostics 2020; 10(22):10092-10105. doi:10.7150/thno.44865. https://www.thno.org/v10p10092.htm

CSE
Wu H, He Y, Wu H, Zhou M, Xu Z, Xiong R, Yan F, Liu H. 2020. Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes. Theranostics. 10(22):10092-10105.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use