Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2016; 6(7):1012-1022. doi:10.7150/thno.15095 This issue Cite

Research Paper

A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles

Hao Zhou1, Zhiyuan Fan1, Pelin K. Lemons1, Hao Cheng1,2✉

1. Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USA;
2. School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania, USA.

✉ Corresponding author: hchengedu.

Citation:
Zhou H, Fan Z, Lemons PK, Cheng H. A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles. Theranostics 2016; 6(7):1012-1022. doi:10.7150/thno.15095. https://www.thno.org/v06p1012.htm
Other styles

File import instruction

Abstract

Graphic abstract

Convenient strategies to provide cell membrane-coated nanoparticles (CM-NPs) with multi-functionalities beyond the natural function of cell membranes would dramatically expand the application of this emerging class of nanomaterials. We have developed a facile approach to functionalize CM-NPs by chemically modifying live cell membranes prior to CM-NP fabrication using a bifunctional linker, succinimidyl-[(N-maleimidopropionamido)-polyethyleneglycol] ester (NHS-PEG-Maleimide). This method is particularly suitable to conjugate large bioactive molecules such as proteins on cell membranes as it establishes a strong anchorage and enable the control of linker length, a critical parameter for maximizing the function of anchored proteins. As a proof of concept, we show the conjugation of human recombinant hyaluronidase, PH20 (rHuPH20) on red blood cell (RBC) membranes and demonstrate that long linker (MW: 3400) is superior to short linker (MW: 425) for maintaining enzyme activity, while minimizing the changes to cell membranes. When the modified membranes were fabricated into RBC membrane-coated nanoparticles (RBCM-NPs), the conjugated rHuPH20 can assist NP diffusion more efficiently than free rHuPH20 in matrix-mimicking gels and the pericellular hyaluronic acid matrix of PC3 prostate cancer cells. After quenching the unreacted chemical groups with polyethylene glycol, we demonstrated that the rHuPH20 modification does not reduce the ultra-long blood circulation time of RBCM-NPs. Therefore, this surface engineering approach provides a platform to functionlize CM-NPs without sacrificing the natural function of cell membranes.

Keywords: Tumor penetration, extracellular matrix, biomimetics, lymph nodes, drug delivery.

Citation styles

APA
Zhou, H., Fan, Z., Lemons, P.K., Cheng, H. (2016). A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles. Theranostics, 6(7), 1012-1022. https://doi.org/10.7150/thno.15095.

ACS
Zhou, H.; Fan, Z.; Lemons, P.K.; Cheng, H. A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles. Theranostics 2016, 6 (7), 1012-1022. DOI: 10.7150/thno.15095.

NLM
Zhou H, Fan Z, Lemons PK, Cheng H. A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles. Theranostics 2016; 6(7):1012-1022. doi:10.7150/thno.15095. https://www.thno.org/v06p1012.htm

CSE
Zhou H, Fan Z, Lemons PK, Cheng H. 2016. A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles. Theranostics. 6(7):1012-1022.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use