A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging

Wang, Yaping; Lin, Tingting; Zhang, Wenyuan; Jiang, Yifan; Jin, Hongyue; He, Huining; Yang, Victor C.; Chen, Yi; Huang, Yongzhuo

doi:10.7150/thno.11139

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Theranostics 2015; 5(8):787-795. doi:10.7150/thno.11139 This issue Cite

Research Paper

A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging

Yaping Wang^1,2, Tingting Lin^1,2, Wenyuan Zhang², Yifan Jiang², Hongyue Jin², Huining He¹, Victor C. Yang^1,3, Yi Chen², Yongzhuo Huang^2✉

1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Hai-ke Rd, Shanghai 201203, China.
3. University of Michigan, College of Pharmacy, 428 Church St, Ann Arbor, MI 48108, USA.

Citation:

Wang Y, Lin T, Zhang W, Jiang Y, Jin H, He H, Yang VC, Chen Y, Huang Y. A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging. Theranostics 2015; 5(8):787-795. doi:10.7150/thno.11139. https://www.thno.org/v05p0787.htm

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Abstract

Tumor-associated proteases (TAPs) have been intensively studied because of their critical roles in cancer development. As a case in point, expression of matrix metalloproteases (MMP) is significantly up-regulated in tumorigenesis, invasion, and metastasis among a majority of cancers. Here we present a prodrug-type, MMP-2-responsive nanoprobe system with high efficiency and low toxicity for detecting MMP-2-overexpressed tumors. The nanoprobe system is featured by its self-assembled fabrication and FRET effect. This prodrug-type nanoprobe is selectively activated by MMP-2, and thus useful for detection of the MMP-2-overexpressed cells and tumors. The nanoprobe system works successfully in various animal tumor models, including human fibrosarcoma and subcutaneous glioma xenograft. Furthermore, in order to overcome the blood brain barrier (BBB) and achieve brain tumor targeting, a transferrin-receptor targeting peptide (T7 peptide) is strategically incorporated into the nanoprobe. The T7-functionalized nanoprobe is capable of detecting the orthotopic brain tumor, with clear, real-time in vivo imaging. This method is promising for in vivo detection of brain tumor, and real-time monitor of a TAP (i.e., MMP-2).

Keywords: matrix metalloprotease, protease-activatable probe, tumor imaging, brain tumor, fluorescence resonance energy transfer.

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APA

Wang, Y., Lin, T., Zhang, W., Jiang, Y., Jin, H., He, H., Yang, V.C., Chen, Y., Huang, Y. (2015). A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging. Theranostics, 5(8), 787-795. https://doi.org/10.7150/thno.11139.

ACS

Wang, Y.; Lin, T.; Zhang, W.; Jiang, Y.; Jin, H.; He, H.; Yang, V.C.; Chen, Y.; Huang, Y. A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging. Theranostics 2015, 5 (8), 787-795. DOI: 10.7150/thno.11139.

NLM

CSE

Wang Y, Lin T, Zhang W, Jiang Y, Jin H, He H, Yang VC, Chen Y, Huang Y. 2015. A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging. Theranostics. 5(8):787-795.

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