Theranostics 2017; 7(18):4470-4479. doi:10.7150/thno.20616

Research Paper

Characterization of Cardiac Dynamics in an Acute Myocardial Infarction Model by Four-Dimensional Optoacoustic and Magnetic Resonance Imaging

Hsiao-Chun Amy Lin1, 2, Xosé Luís Déan-Ben1, Ivana Ivankovic1, 2, Melanie A. Kimm3, Katja Kosanke3, Helena Haas3, Reinhard Meier3, Fabian Lohöfer3, Moritz Wildgruber2, 3, 4 *, Daniel Razansky1, 2*✉

1. Institute for Biological and Medical Imaging (IBMI), Helmholtz Center Munich, Neuherberg, Germany;
2. Faculty of Medicine, Technical University of Munich, Germany;
3. Department of Diagnostic and Interventional Radiology, Technical University of Munich, Germany;
4. Translational Research Imaging Center, Department of Clinical Radiology, Universitätsklinikum Münster, Germany.
* Equal contribution

Abstract

Extraction of murine cardiac functional parameters on a beat-by-beat basis is limited with the existing imaging modalities due to insufficient three-dimensional temporal resolution. Faster volumetric imaging methods enabling in vivo characterization of functional parameters are poised to advance cardiovascular research and provide a better understanding of the mechanisms underlying cardiac diseases. We present a new approach based on analyzing contrast-enhanced optoacoustic (OA) images acquired at high volumetric frame rate without using cardiac gating or other approaches for motion correction. We apply an acute murine myocardial infarction model optimized for acquisition of artifact-free optoacoustic imaging data to study cardiovascular hemodynamics. Infarcted hearts (n = 21) could be clearly differentiated from healthy controls (n = 9) based on a significantly higher pulmonary transit time (PTT) (2.25 [2.00-2.41] s versus 1.34 [1.25-1.67] s, p = 0.0235), while no statistically significant difference was observed in the heart rate (318 [252-361] bpm versus 264 [252-320] bpm, p = 0.3129). Nevertheless, nonlinear heartbeat dynamics was stronger in the healthy hearts, as evidenced by the third harmonic component in the heartbeat spectra. MRI data acquired from the same mice further revealed that the PTT increases with the size of infarction and similarly increases with reduced ejection fraction. Moreover, an inverse relationship between infarct PTT and time post-surgery was found, which suggests the occurrence of cardiac healing. In combination with the proven ability of optoacoustics to track targeted probes within the injured myocardium, our method can depict cardiac anatomy, function, and molecular signatures, with both high spatial and temporal resolution. Volumetric four-dimensional optoacoustic characterization of cardiac dynamics with supreme temporal resolution can capture cardiovascular dynamics on a beat-by-beat basis in mouse models of myocardial ischemia.

Keywords: acute myocardial infarction, real-time cardiac imaging, photoacoustics, heart rate, optoacoustic imaging, pulmonary transit time.

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How to cite this article:
Lin HCA, Déan-Ben XL, Ivankovic I, Kimm MA, Kosanke K, Haas H, Meier R, Lohöfer F, Wildgruber M, Razansky D. Characterization of Cardiac Dynamics in an Acute Myocardial Infarction Model by Four-Dimensional Optoacoustic and Magnetic Resonance Imaging. Theranostics 2017; 7(18):4470-4479. doi:10.7150/thno.20616. Available from http://www.thno.org/v07p4470.htm