Theranostics 2017; 7(9):2377-2391. doi:10.7150/thno.19408

Research Paper

An [18F]-Positron-Emitting, Fluorescent, Cerebrospinal Fluid Probe for Imaging Damage to the Brain and Spine

Harikrishna Kommidi1, Hua Guo1, Nandi Chen1, 2, Dohyun Kim3, Bin He3, Amy P. Wu4, Omer Aras5✉, Richard Ting1✉

1. Department of Radiology, Molecular Imaging Innovations Institute (MI3), Weill Cornell Medical College, New York, NY 10065, USA;
2. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China;
3. Department of Radiology, Weill Cornell Medical College, New York, NY 10065, USA;
4. Department of Otolaryngology - Head & Neck Surgery, Northwell Health, Hofstra Northwell School of Medicine. New York, NY, 10075, USA;
5. Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Abstract

Fluorescein is modified to bear 18F so that it can act as both a positron emitter, and a fluorophore, allowing detection by positron emission tomography (PET), scintillation, and fluorescent imaging (FL). [18F]-2 is injected into the intrathecal space of rats and used to observe the cerebrospinal fluid (CSF) that bathes the brain and spine. Injury in three different applications is visualized with [18F]-2: 1) detection of a 0.7 mm paranasal-sinus CSF leak (CSFL); 2) detection of 0.5 mm puncture damage to the thoracic spine (acute spinal cord injury); and 3) detection of intracerebral hemorrhage/edema because of traumatic brain injury. In all models, the location of injury is visualized with [18F]-2 at high resolution. [18F]-2 PET imaging may be a superior alternative to current clinical contrast myelography and 131I, 111In or 99mTc radionuclide cisternography. Like fluorescein, [18F]-2 may also have other uses in diagnostic or fluorescence guided medicine.

Keywords: Positron emission tomography, imaging, optical imaging, radionuclide cisternography, myelography.

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How to cite this article:
Kommidi H, Guo H, Chen N, Kim D, He B, Wu AP, Aras O, Ting R. An [18F]-Positron-Emitting, Fluorescent, Cerebrospinal Fluid Probe for Imaging Damage to the Brain and Spine. Theranostics 2017; 7(9):2377-2391. doi:10.7150/thno.19408. Available from http://www.thno.org/v07p2377.htm