Theranostics 2016; 6(10):1683-1702. doi:10.7150/thno.15850


Optical Aptasensors for Adenosine Triphosphate

Stella Ng, Hui Si Lim, Qian Ma, Zhiqiang Gao

Department of Chemistry, National University of Singapore, Singapore 117543.


Nucleic acids are among the most researched and applied biomolecules. Their diverse two- and three-dimensional structures in conjunction with their robust chemistry and ease of manipulation provide a rare opportunity for sensor applications. Moreover, their high biocompatibility has seen them being used in the construction of in vivo assays. Various nucleic acid-based devices have been extensively studied as either the principal element in discrete molecule-like sensors or as the main component in the fabrication of sensing devices. The use of aptamers in sensors - aptasensors, in particular, has led to improvements in sensitivity, selectivity, and multiplexing capacity for a wide verity of analytes like proteins, nucleic acids, as well as small biomolecules such as glucose and adenosine triphosphate (ATP). This article reviews the progress in the use of aptamers as the principal component in sensors for optical detection of ATP with an emphasis on sensing mechanism, performance, and applications with some discussion on challenges and perspectives.

Keywords: Adenosine triphosphate, nucleic acid, aptamers, aptasensors, fluorometry, colorimetry, chemiluminescence.

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How to cite this article:
Ng S, Lim HS, Ma Q, Gao Z. Optical Aptasensors for Adenosine Triphosphate. Theranostics 2016; 6(10):1683-1702. doi:10.7150/thno.15850. Available from