Theranostics 2016; 6(10):1603-1610. doi:10.7150/thno.15534

Research Paper

Holographic Assessment of Lymphoma Tissue (HALT) for Global Oncology Field Applications

Divya Pathania1,2*, Hyungsoon Im1,2*, Aoife Kilcoyne1,2, Aliyah R. Sohani3, Lioubov Fexon1, Misha Pivovarov1,2, Jeremy S. Abramson4,5,6, Thomas C. Randall5,7,8, Bruce A. Chabner5,6, Ralph Weissleder1,2,9✉, Hakho Lee1,2✉, Cesar M. Castro1,5,6✉

1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
3. Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114;
4. Center for Lymphoma, Massachusetts General Hospital, Boston, MA, 02114;
5. Massachusetts General Hospital Cancer Center, Boston, MA 02114;
6. Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
7. Global Oncology Initiative, Dana-Farber / Harvard Cancer Center, Boston, MA, 02115;
8. Department of Obstetrics, Gynecology and Reproductive Biology, Massachusetts General Hospital, Boston, MA 02114;
9. Department of Systems Biology, Harvard Medical School, Boston, MA 02115.
* These authors contributed equally to the paper.

Abstract

Low-cost, rapid and accurate detection technologies are key requisites to cope with the growing global cancer challenges. The need is particularly pronounced in resource-limited settings where treatment opportunities are often missed due to the absence of timely diagnoses. We herein describe a Holographic Assessment of Lymphoma Tissue (HALT) system that adopts a smartphone as the basis for molecular cancer diagnostics. The system detects malignant lymphoma cells labeled with marker-specific microbeads that produce unique holographic signatures. Importantly, we optimized HALT to detect lymphomas in fine-needle aspirates from superficial lymph nodes, procedures that align with the minimally invasive biopsy needs of resource-constrained regions. We equipped the platform to directly address the practical needs of employing novel technologies for “real world” use. The HALT assay generated readouts in <1.5 h and demonstrated good agreement with standard cytology and surgical pathology.

Keywords: Cancer diagnostics, Smartphone testing, Point-of-care diagnostics, global oncology.

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How to cite this article:
Pathania D, Im H, Kilcoyne A, Sohani AR, Fexon L, Pivovarov M, Abramson JS, Randall TC, Chabner BA, Weissleder R, Lee H, Castro CM. Holographic Assessment of Lymphoma Tissue (HALT) for Global Oncology Field Applications. Theranostics 2016; 6(10):1603-1610. doi:10.7150/thno.15534. Available from http://www.thno.org/v06p1603.htm