Theranostics 2015; 5(4):431-442. doi:10.7150/thno.10891

Research Paper

Cancer Cell-Specific Oligopeptides Selected by an Integrated Microfluidic System from a Phage Display Library for Ovarian Cancer Diagnosis

Chih-Hung Wang1, Chen-Hsun Weng1, Yu-Jui Che1, Kuan Wang4, Gwo-Bin Lee1, 2, 3 ✉

1. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
2. Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan
3. Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan
4. Nanomedicine Program and Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

Abstract

Ovarian cancer is one of the leading causes of female mortality worldwide. Unfortunately, there are currently few high-specificity candidate oligopeptide targeting agents that can be used for early diagnosis of this cancer. It has been suggested that cancer-specific oligopeptides could be screened from a phage display library. However, conventional methods are tedious, labor-intensive, and time consuming. Therefore, a novel, integrated microfluidic system was developed to automate the entire screening process for ovarian cancer cell-specific oligopeptides. An oligopeptide screened with microfluidic chip-based technique was demonstrated to have high affinity to ovarian cancer cells and demonstrated relatively low binding to other cancer cells, indicating a high specificity. Furthermore, the developed method consumed relatively low volumes of samples and reagents; only 70 μL of reactant was used within the whole experimental process. Each panning process was also significantly shortened to only 7.5 hours. Therefore, the screened oligopeptide could be used to isolate ovarian cancer cells in a rapid manner, thus greatly expediting the diagnosis and its application as oligopeptide targeting agent for theranostics of this cancer.

Keywords: microfluidic chip, oligopeptide targeting agent, ovarian cancer, phage display library

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How to cite this article:
Wang CH, Weng CH, Che YJ, Wang K, Lee GB. Cancer Cell-Specific Oligopeptides Selected by an Integrated Microfluidic System from a Phage Display Library for Ovarian Cancer Diagnosis. Theranostics 2015; 5(4):431-442. doi:10.7150/thno.10891. Available from http://www.thno.org/v05p0431.htm