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Theranostics 2018; 8(7):1911-1922. doi:10.7150/thno.22534 This issue Cite

Research Paper

Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection

Tang Gao1, Hongliang Zeng2, Huan Xu3, Feng Gao3, Wei Li3, Shengwang Zhang3, Yi Liu1, Guifang Luo2, Mingdan Li2, Dejian Jiang2, Zhigao Chen2, Yong Wu3, Wei Wang3, Wenbin Zeng1,✉

1. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
2. Hunan Provincial Research Center for Safety Evaluation of Drugs, Liuyang National Economic and Technical Development Zone 410331, China.
3. The Third Xiangya Hospital, Central South University.

✉ Corresponding author: Prof Dr Wenbin Zeng, Tel(Fax): 0086-731-82650459; E-mail address: wbzengcom (W. Zeng)

Citation:
Gao T, Zeng H, Xu H, Gao F, Li W, Zhang S, Liu Y, Luo G, Li M, Jiang D, Chen Z, Wu Y, Wang W, Zeng W. Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection. Theranostics 2018; 8(7):1911-1922. doi:10.7150/thno.22534. https://www.thno.org/v08p1911.htm
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Abstract

Graphic abstract

Background: Increasing bacterial infections as well as a rise in bacterial resistance call for the development of novel and safe antimicrobial agents without inducing bacterial resistance. Nanoparticles (NPs) present some advantages in treating bacterial infections and provide an alternative strategy to discover new antibiotics. Here, we report the development of novel self-assembled fluorescent organic nanoparticles (FONs) with excellent antibacterial efficacy and good biocompatibility.

Methods: Self-assembly of 1-(12-(pyridin-1-ium-1-yl)dodecyl)-4-(1,4,5-triphenyl-1H-imidazol-2-yl)pyridin-1-ium (TPIP) in aqueous solution was investigated using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The bacteria were imaged under a laser scanning confocal microscope. We evaluated the antibacterial efficacy of TPIP-FONs in vitro using sugar plate test. The antimicrobial mechanism was explored by SEM. The biocompatibility of the nanoparticles was examined using cytotoxicity test, hemolysis assay, and histological staining. We further tested the antibacterial efficacy of TPIP-FONs in vivo using the S. aureus-infected rats.

Results: In aqueous solution, TPIP could self-assemble into nanoparticles (TPIP-FONs) with characteristic aggregation-induced emission (AIE). TPIP-FONs could simultaneously image gram-positive bacteria without the washing process. In vitro antimicrobial activity suggested that TPIP-FONs had excellent antibacterial activity against S. aureus (MIC = 2.0 µg mL-1). Furthermore, TPIP-FONs exhibited intrinsic biocompatibility with mammalian cells, in particular, red blood cells. In vivo studies further demonstrated that TPIP-FONs had excellent antibacterial efficacy and significantly reduced bacterial load in the infectious sites.

Conclusion: The integrated design of bacterial imaging and antibacterial functions in the self-assembled small molecules provides a promising strategy for the development of novel antimicrobial nanomaterials.

Keywords: antibacterial materials, self-assembly, aggregation-induced emission, bacterial imaging, antimicrobial activity.

Citation styles

APA
Gao, T., Zeng, H., Xu, H., Gao, F., Li, W., Zhang, S., Liu, Y., Luo, G., Li, M., Jiang, D., Chen, Z., Wu, Y., Wang, W., Zeng, W. (2018). Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection. Theranostics, 8(7), 1911-1922. https://doi.org/10.7150/thno.22534.

ACS
Gao, T.; Zeng, H.; Xu, H.; Gao, F.; Li, W.; Zhang, S.; Liu, Y.; Luo, G.; Li, M.; Jiang, D.; Chen, Z.; Wu, Y.; Wang, W.; Zeng, W. Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection. Theranostics 2018, 8 (7), 1911-1922. DOI: 10.7150/thno.22534.

NLM
Gao T, Zeng H, Xu H, Gao F, Li W, Zhang S, Liu Y, Luo G, Li M, Jiang D, Chen Z, Wu Y, Wang W, Zeng W. Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection. Theranostics 2018; 8(7):1911-1922. doi:10.7150/thno.22534. https://www.thno.org/v08p1911.htm

CSE
Gao T, Zeng H, Xu H, Gao F, Li W, Zhang S, Liu Y, Luo G, Li M, Jiang D, Chen Z, Wu Y, Wang W, Zeng W. 2018. Novel Self-assembled Organic Nanoprobe for Molecular Imaging and Treatment of Gram-positive Bacterial Infection. Theranostics. 8(7):1911-1922.

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