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Theranostics 2016; 6(8):1105-1118. doi:10.7150/thno.14700 This issue Cite

Research Paper

MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma

Alessia Lo Dico1,9*, Viviana Costa2*, Cristina Martelli3, Cecilia Diceglie3,4, Francesca Rajata5, Aroldo Rizzo5, Carmine Mancone6, Marco Tripodi6,7, Luisa Ottobrini3,8, Riccardo Alessandro9,10#✉, Alice Conigliaro6#✉

1. Tecnomed Foundation of the University of Milano-Bicocca, Monza 20900, Italy
2. Laboratory of Tissue Engineering - Innovative Technology Platforms for Tissue Engineering (PON01-00829), Rizzoli Orthopedic Institute, Palermo 90127, Italy
3. Department of Pathophysiology and Transplantation, University of Milan, Milan 20100, Italy;
4. Doctorate School of Molecular Medicine, University of Milan, Milan 20100, Italy;
5. Unità Operativa di Anatomia Patologica, Azienda Ospedaliera Ospedali Riuniti "Villa Sofia-Cervello", Palermo 90100, Italy;
6. Dipartimento di Biotecnologie Cellulari ed Ematologia, Sapienza University of Rome, Rome 00185, Italy;
7. National Institute for Infectious Diseases L. Spallanzani, IRCCS, Rome 00149, Italy;
8. Institute of Molecular Bioimaging and Physiology (IBFM), National Researches Council (CNR), Segrate (MI) 20093, Italy;
9. Dipartimento di Biopatologia e Biotecnologie Mediche, University of Palermo, Palermo 90127, Italy;
10. Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council of Italy, Palermo 90146, Italy.
* these authors contributed equally to this work.
# joint senior authors.

✉ Corresponding authors: Alice Conigliaro (conigliarouniroma1.it); Riccardo Alessandro (riccardo.alessandroit).

Citation:
Lo Dico A, Costa V, Martelli C, Diceglie C, Rajata F, Rizzo A, Mancone C, Tripodi M, Ottobrini L, Alessandro R, Conigliaro A. MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics 2016; 6(8):1105-1118. doi:10.7150/thno.14700. https://www.thno.org/v06p1105.htm
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Abstract

Graphic abstract

Hypoxia is a common feature in solid tumours. In glioma, it is considered the major driving force for tumour angiogenesis and correlates with enhanced resistance to conventional therapies, increased invasiveness and a poor prognosis for patients. Here we describe, for the first time, that miR675-5p, embedded in hypoxia-induced long non-coding RNA H19, plays a mandatory role in establishing a hypoxic response and in promoting hypoxia-mediated angiogenesis. We demonstrated, in vitro and in vivo, that miR675-5p over expression in normoxia is sufficient to induce a hypoxic moreover, miR675-5p depletion in low oxygen conditions, drastically abolishes hypoxic responses including angiogenesis. In addition, our data indicate an interaction of miR675-5p, HIF-1α mRNA and the RNA Binding Protein HuR in hypoxia-induced responses. We suggest the modulation of miR675-5p as a new therapeutic option to promote or abolish hypoxia induced angiogenesis.

Keywords: Angiogenesis, hypoxia, glioma, miRNA675, optical imaging, HuR, VHL.

Citation styles

APA
Lo Dico, A., Costa, V., Martelli, C., Diceglie, C., Rajata, F., Rizzo, A., Mancone, C., Tripodi, M., Ottobrini, L., Alessandro, R., Conigliaro, A. (2016). MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics, 6(8), 1105-1118. https://doi.org/10.7150/thno.14700.

ACS
Lo Dico, A.; Costa, V.; Martelli, C.; Diceglie, C.; Rajata, F.; Rizzo, A.; Mancone, C.; Tripodi, M.; Ottobrini, L.; Alessandro, R.; Conigliaro, A. MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics 2016, 6 (8), 1105-1118. DOI: 10.7150/thno.14700.

NLM
Lo Dico A, Costa V, Martelli C, Diceglie C, Rajata F, Rizzo A, Mancone C, Tripodi M, Ottobrini L, Alessandro R, Conigliaro A. MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics 2016; 6(8):1105-1118. doi:10.7150/thno.14700. https://www.thno.org/v06p1105.htm

CSE
Lo Dico A, Costa V, Martelli C, Diceglie C, Rajata F, Rizzo A, Mancone C, Tripodi M, Ottobrini L, Alessandro R, Conigliaro A. 2016. MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics. 6(8):1105-1118.

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