Theranostics 2016; 6(6):828-836. doi:10.7150/thno.14695

Research Paper

Efficient Capture and Isolation of Tumor-Related Circulating Cell-Free DNA from Cancer Patients Using Electroactive Conducting Polymer Nanowire Platforms

SeungHyun Jeon1,3*, HyungJae Lee1,4,*, Kieun Bae2, Kyong-Ah Yoon2,5, Eun Sook Lee1, Youngnam Cho1✉

1. New Experimental Therapeutic Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;
2. Lung Cancer Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;
3. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine;
4. Department of Medical Science, Yonsei University College of Medicine, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea;
5. College of Veterinary Medicine, Konkuk University, Seoul, Korea.
* These authors contributed equally.

Abstract

Circulating cell-free DNA (cfDNA) is currently recognized as a key non-invasive biomarker for cancer diagnosis and progression and therapeutic efficacy monitoring. Because cfDNA has been detected in patients with diverse types of cancers, the use of efficient strategies to isolate cfDNA not only provides valuable insights into tumour biology, but also offers the potential for developing new cancer-specific targets. However, the challenges associated with conventional cfDNA extraction methods prevent their further clinical applications. Here, we developed a nanostructured conductive polymer platform for the efficient capture and release of circulating cfDNA and demonstrated its potential clinical utility using unprocessed plasma samples from patients with breast and lung cancers. Our results confirmed that the platform's enhanced efficiency allows tumor-specific circulating cfDNA to be recovered at high yield and purity.

Keywords: cancer, circulating cell-free DNA, liquid biopsy, conducting polymer, nanowired structure, mutation detection.

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How to cite this article:
Jeon S, Lee H, Bae K, Yoon KA, Lee ES, Cho Y. Efficient Capture and Isolation of Tumor-Related Circulating Cell-Free DNA from Cancer Patients Using Electroactive Conducting Polymer Nanowire Platforms. Theranostics 2016; 6(6):828-836. doi:10.7150/thno.14695. Available from http://www.thno.org/v06p0828.htm