Theranostics 2016; 6(2):155-166. doi:10.7150/thno.13536

Research Paper

A Fluorescence-Guided Laser Ablation System for Removal of Residual Cancer in a Mouse Model of Soft Tissue Sarcoma

Alexander L. Lazarides1*, Melodi J. Whitley1,2*, David B. Strasfeld3, Diana M. Cardona4, Jorge M. Ferrer3, Jenna L. Mueller5, Henry L. Fu5, Suzanne Bartholf DeWitt6, Brian E. Brigman6, Nimmi Ramanujam5, David G. Kirsch2,7, William C. Eward6✉

1. School of Medicine, Duke University, Durham, North Carolina
2. Department of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, North Carolina
3. Lumicell, Inc., Wellesley, MA
4. Department of Pathology, Duke University Medical Center, Durham, North Carolina
5. Department of Biomedical Engineering, Duke University, Durham, North Carolina
6. Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina
7. Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina
* Co-first authors

Abstract

The treatment of soft tissue sarcoma (STS) generally involves tumor excision with a wide margin. Although advances in fluorescence imaging make real-time detection of cancer possible, removal is limited by the precision of the human eye and hand. Here, we describe a novel pulsed Nd:YAG laser ablation system that, when used in conjunction with a previously described molecular imaging system, can identify and ablate cancer in vivo. Mice with primary STS were injected with the protease-activatable probe LUM015 to label tumors. Resected tissues from the mice were then imaged and treated with the laser using the paired fluorescence-imaging/ laser ablation device, generating ablation clefts with sub-millimeter precision and minimal underlying tissue damage. Laser ablation was guided by fluorescence to target tumor tissues, avoiding normal structures. The selective ablation of tumor implants in vivo improved recurrence-free survival after tumor resection in a cohort of 14 mice compared to 12 mice that received no ablative therapy. This prototype system has the potential to be modified so that it can be used during surgery to improve recurrence-free survival in patients with cancer.

Keywords: Fluorescence Imaging, Laser Ablation, Sarcoma, Local Neoplasm Recurrence, Image-Guided Surgery

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How to cite this article:
Lazarides AL, Whitley MJ, Strasfeld DB, Cardona DM, Ferrer JM, Mueller JL, Fu HL, DeWitt SB, Brigman BE, Ramanujam N, Kirsch DG, Eward WC. A Fluorescence-Guided Laser Ablation System for Removal of Residual Cancer in a Mouse Model of Soft Tissue Sarcoma. Theranostics 2016; 6(2):155-166. doi:10.7150/thno.13536. Available from http://www.thno.org/v06p0155.htm