Theranostics
2014; 4(10):1039-1051.
doi:10.7150/thno.7866 This issueCite
Research Paper
Megalin-Mediated Specific Uptake of Chitosan/siRNA Nanoparticles in Mouse Kidney Proximal Tubule Epithelial Cells Enables AQP1 Gene Silencing
Shan Gao1*, San Hein1#, Frederik Dagnæs-Hansen2, Kathrin Weyer2,3, Chuanxu Yang1, Rikke Nielsen2,3, Erik I Christensen2,3, Robert A Fenton2,3,4✉, Jørgen Kjems1✉
1. The Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark. 2. Department of Biomedicine, Aarhus University, Aarhus C, Denmark; 3. MEMBRANES, Aarhus University, Aarhus C, Denmark; 4. Center for Interactions of Proteins in Epithelial Transport, Aarhus University, Aarhus C, Denmark. *Present address: Kunshan RNAi Institute, Kunshan, Jiangsu 215300, China. #Present address: Water Security Corporation, 1455 Kleppe Lane, Sparks NV 89501, USA.
✉ Corresponding authors: J.K.: Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. Email: jkau.dk; Telephone: 0045 87155494; Fax: 0045 86196500. OR R.A.F.: Department of Biomedicine - Anatomy, Aarhus University, Wilhelm Meyers Allé 3, building 1233, Aarhus C, Denmark. Email: rofeau.dk; Telephone: 0045 87167671; Fax: 0045 871 67102.More
Citation:
Gao S, Hein S, Dagnæs-Hansen F, Weyer K, Yang C, Nielsen R, Christensen EI, Fenton RA, Kjems J. Megalin-Mediated Specific Uptake of Chitosan/siRNA Nanoparticles in Mouse Kidney Proximal Tubule Epithelial Cells Enables AQP1 Gene Silencing. Theranostics 2014; 4(10):1039-1051. doi:10.7150/thno.7866. https://www.thno.org/v04p1039.htm
RNAi-based strategies provide a great therapeutic potential for treatment of various human diseases including kidney disorders, but face the challenge of in vivo delivery and specific targeting. The chitosan delivery system has previously been shown to target siRNA specifically to the kidneys in mice when administered intravenously. Here we confirm by 2D and 3D bioimaging that chitosan formulated siRNA is retained in the kidney for more than 48 hours where it accumulates in proximal tubule epithelial cells (PTECs), a process that was strongly dependent on the molecular weight of chitosan. Chitosan/siRNA nanoparticles, administered to chimeric mice with conditional knockout of the megalin gene, distributed almost exclusively in cells that expressed megalin, implying that the chitosan/siRNA particle uptake was mediated by a megalin-dependent endocytotic pathway. Knockdown of the water channel aquaporin 1 (AQP1) by up to 50% in PTECs was achieved utilizing the systemic i.v. delivery of chitosan/AQP1 siRNA in mice. In conclusion, specific targeting PTECs with the chitosan nanoparticle system may prove to be a useful strategy for knockdown of specific genes in PTECs, and provides a potential therapeutic strategy for treating various kidney diseases.