Theranostics 2014; 4(2):142-153. doi:10.7150/thno.7266

Research Paper

Folate Receptor-Targeting Gold Nanoclusters as Fluorescence Enzyme Mimetic Nanoprobes for Tumor Molecular Colocalization Diagnosis

Dehong Hu*, Zonghai Sheng*, Shengtao Fang, Yanan Wang, Duyang Gao, Pengfei Zhang, Ping Gong, Yifan Ma, Lintao Cai

Guangdong Key Laboratory of Nanomedicine, CAS Key Laboratory of Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China
* Dehong Hu and Zonghai Sheng equally contributed to this work.

Abstract

Nanoprobes with enzyme-like properties attracted a growing interest in early screening and diagnosis of cancer. To achieve high accuracy and specificity of tumor detection, the design and preparation of enzyme mimetic nanoprobes with high enzyme activity, tumor targeting and excellent luminescence property is highly desirable. Herein, we described a novel kind of fluorescence enzyme mimetic nanoprobe based on folate receptor-targeting Au nanoclusters. The nanoprobes exhibited excellent stability, low cytotoxicity, high fluorescence and enzyme activity. We demonstrated that the nanoprobes could be used for tumor tissues fluorescence/visualizing detection. For the same tumor tissue slice, the nanoprobes peroxidase staining and fluorescent staining were obtained simultaneously, and the results were mutually complementary. Therefore, the fluorescence enzyme mimetic nanoprobes could provide a molecular colocalization diagnosis strategy, efficiently avoid false-positive and false-negative results, and further improve the accuracy and specificity of cancer diagnoses. By examining different clinical samples, we demonstrated that the nanoprobes could distinguish efficiently cancerous cells from normal cells, and exhibit a clinical potential for cancer diagnosis.

Keywords: Gold Nanoclusters, Fluorescence, Enzyme Mimetic, Tumor Diagnosis, Molecular Colocalization

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How to cite this article:
Hu D, Sheng Z, Fang S, Wang Y, Gao D, Zhang P, Gong P, Ma Y, Cai L. Folate Receptor-Targeting Gold Nanoclusters as Fluorescence Enzyme Mimetic Nanoprobes for Tumor Molecular Colocalization Diagnosis. Theranostics 2014; 4(2):142-153. doi:10.7150/thno.7266. Available from http://www.thno.org/v04p0142.htm