Theranostics 2013; 3(5):317-330. doi:10.7150/thno.5284

Review

Upconversion Nanoparticles for Photodynamic Therapy and Other Cancer Therapeutics

Chao Wang, Liang Cheng, Zhuang Liu Corresponding address

Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.

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How to cite this article:
Wang C, Cheng L, Liu Z. Upconversion Nanoparticles for Photodynamic Therapy and Other Cancer Therapeutics. Theranostics 2013; 3(5):317-330. doi:10.7150/thno.5284. Available from http://www.thno.org/v03p0317.htm

Abstract

Photodynamic therapy (PDT) is a non-invasive treatment modality for a variety of diseases including cancer. PDT based on upconversion nanoparticles (UCNPs) has received much attention in recent years. Under near-infrared (NIR) light excitation, UCNPs are able to emit high-energy visible light, which can activate surrounding photosensitizer (PS) molecules to produce singlet oxygen and kill cancer cells. Owing to the high tissue penetration ability of NIR light, NIR-excited UCNPs can be used to activate PS molecules in much deeper tissues compared to traditional PDT induced by visible or ultraviolet (UV) light. In addition to the application of UCNPs as an energy donor in PDT, via similar mechanisms, they could also be used for the NIR light-triggered drug release or activation of 'caged' imaging or therapeutic molecules. In this review, we will summarize the latest progresses regarding the applications of UCNPs for photodynamic therapy, NIR triggered drug and gene delivery, as well as several other UCNP-based cancer therapeutic approaches. The future prospects and challenges in this emerging field will be also discussed.

Keywords: Photodynamic therapy, Upconversion nanoparticles.

1. Introduction

In the past few decades, photodynamic therapy (PDT) has emerged as alternative treatment approach to chemotherapy and radiotherapy to treat various diseases including cancer. PDT is considered to be minimally invasive compared with chemotherapy and radiotherapy.[1] Three key components are involved in a typical PDT process: light, photosensitizer (PS) molecules, and oxygen. With the absorption of light, a ground state PS is boosted into a high-energy state, leading to energy transfer to neighboring oxygen or other substrate molecules, and then the generation of singlet oxygen (1O2) or other reactive oxygen species (ROS). The ROS species produced during PDT are able to destroy tumors by multifactorial mechanisms, including directly inducing cancer cell death by necrosis and/or apoptosis,[2] destruction of tumor vasculatures as an anti-angiogenesis effect,[3] and also the stimulation of the host immune system to recognize, track down and destroy any remaining tumor cells, in contrast to radiotherapy and chemotherapy which are mostly immunosuppressive.[4] Moreover, in common with other local cancer therapies such as cryotherapy and hyperthermia, PDT can be locally applied onto a specific region by selectively illuminating the lesion such as the tumor by light, while leaving normal tissues untouched, thus offering much lower toxic side effects compared to the radiotherapy and chemotherapy which kill normal cells together with cancer cells in a less selective manner.

Despite the above-mentioned advantages of PDT, one major limitation of this treatment methodology in clinical applications is the poor tissue penetration ability of the light used to activate PS molecules. In fact, most PS molecules currently used in PDT are excited by visible or even UV light, which exhibit rather limited penetration depth in biological tissues, thus hampering the application of PDT in the treatment of large or internal tumors. Therefore, significant efforts have been devoted to develop new PDT strategies induced by NIR light, whose wavelengths fall into the 'transparency window' of biological tissues. It is believed that NIR light (typically in the 700-1000 nm spectral range) not only can afford penetration depths of an order of magnitude greater than that of visible light, but also with a low phototoxicity to normal cells and tissues, and thus is the ideal light source in phototherapies.[5, 6]

Two-photon excited PDT is a new technique developed in recent years that uses NIR light to induce PDT.[7-10] In this approach, a PS molecule, which usually has to be excited by high-energy visible light, can be excited by simultaneous absorption of two NIR low energy photons into the high-energy state to trigger single oxygen generation. However, this technique requires the use of a pulsed laser as the light source to excite focused small areas to obtain sufficient instant energy that needed for two-photon excitation, and thus has limited value for in vivo experiments, not to mention clinical applications.

Another new approach to deliver light into deeper tissues for PDT treatment is using NIR-excitable upconversion nanoparticles (UCNPs) as an energy donor.[11, 12] UCNPs are usually lanthanide-doped nanocrystals, which emit high energy photons under excitation by the NIR light, and have found potential applications in many different fields including biomedicine. A number of different approaches, mainly including thermal decomposition method and hydrothermal method, have been developed to synthesize UCNP nanocrystals with varied sizes and emission spectra[13]. Compared with traditional down-conversion fluorescence, the NIR light excited upconversion luminescence (UCL) of UCNPs exhibits improved tissue penetration depth, higher photochemical stability, and free of auto-fluorescence background[13-22]. Owing to those unique advantages, UCNPs have been widely explored as novel nano-probes in biomedical imaging in recent years. The absence of auto-fluorescence background in UCL of UCNPs offers remarkably improved detection sensitivity, beneficial for highly sensitive in vivo biomedical imaging and cell tracking[23-27]. The UCL emission spectra could be easily tuned via either changing of doping lanthanide ions, or luminescence resonance energy transfer (LRET) by attaching fluorescent dyes or quantum dots (QDs)[28-30]. By developing UCNPs with multiple imaging functions, the UCL imaging may be combined with other imaging modalities such as magnetic resonance (MR) imaging, positron emission tomography (PET), and computer tomography (CT), for multimodal biomedical imaging using a single UCNP-based imaging probe, to overcome the limitations of each single imaging methodology.[31-36] Moreover, therapeutic applications of UCNPs, especially UCNP-based PDT, have also aroused significant interests recently.

Different from the two-photon excited PDT which requires the pulsed laser with high instant energy as the excitation light and can only treat small areas using the focused laser beam, continuous lasers with much lower energy density can be applied to illuminate large areas in UCNP-based PDT. In the past few years, a large number of groups have demonstrated UCNP-based PDT in vitro as well as in vivo. In addition, UCNPs as energy donors can be used for the NIR light-triggered release or 'uncaging' of functional bio-molecules. Several other UCNP-based therapeutic approaches, including imaging-guided drug delivery and photothermal therapy, have also been presented by several different groups. Although there have been a number of recently published articles to review the use of UCNPs in biomedicine, most of them are focused on their synthesis, functionalization, as well as applications in biological sensing and imaging[13, 16, 19]. In this article, while mainly reviewing the development of UCNP-based PDT in details, we will also summarize recent reports regarding the involvements of UCNPs in other cancer therapeutic applications, and discuss the future challenges and promises of using UCNPs as novel theranostic nano-platforms.

2. Loading of photosensitizers on upconversion nanoparticles

Appropriate loading of PS molecules on to UCNPs is important to allow effective resonance energy transfer from donors to acceptors in NIR-induced PDT using UCNP-PS nanocomplexes. Surveying literature, there have been three commonly used approaches to load PS molecules onto UCNPs, including silica encapsulation, non-covalently physical adsorption, and covalent conjugation via chemical linkages (Table 1).

2.1 Silica encapsulation

The first report of using UCNPs for PDT application was published by Zhang et al.[37] In their study, UCNPs were coated with a silica shell, into which PS molecules were doped. Since then, a number of papers have reported similar strategies for the PS loading on UCNPs.[38-46] The team of Zhang [46] developed NaYF4 UCNPs as carriers for zinc(II)-phtalocyanine (ZnPc) (Figure 1a). Instead of doping PS molecules into the silica shell during nanoparticle synthesis, in their system, UCNPs were coated with a layer of mesoporous silica, into which ZnPc molecules were encapsulated by physical adsorption. They demonstrated that the ZnPc molecules loaded into the porous silica shell of nanoparticles were quite stable without being released in phosphate-buffered saline (PBS) solution and RPMI 1640 cell culture medium. Under 980-nm NIR laser excitation, those nanoparticles could continuously produce singlet oxygen for PDT cancer cell killing. This silica encapsulation strategy, although demands certain efforts in nanomaterials fabrication, is likely the most widely adopted method to prepare UCNP-based PDT agents.

2.2 Non-covalent physical adsorption

Non-covalent physical adsorption, as a very simple method to load PS molecules on UCNPs, has been proposed by our group.[47, 48] Since as-prepared UCNPs were capped by oleic acid and not water soluble, a polyethylene glycol (PEG) drafted amphiphilic polymer was used to transfer hydrophobic UCNPs into the aqueous phase, yielding PEGylated UCNPs with excellent water solubility. PEGylated UCNPs prepared by our method contained a hydrophobic oleic acid layer on top of the inorganic nanoparticle surface and beneath the PEG coating. Hydrophobic molecules such as de-protonated doxorubicin (DOX), a chemotherapy drug, and chlorine 6 (Ce6), a PS molecule, could thus be adsorbed into the hydrophobic layer for effective drug loading. The terminals on PEG chains were available for conjugation of targeting ligands such as folate acid for targeted delivery. Our strategy is rather straightforward without the need of any surface reaction and allows the close binding between the nanoparticle and Ce6 molecules, offering effective resonance energy transfer from UCNPs to Ce6. (Figure 1b)

 Table 1 

A summary of published efforts on the development of UCNP-based PDT.

Theranostics Image (Click on the image to enlarge.)
 Figure 1 

Different strategies to load PS molecules on UCNPs. (a) A schematic image showing loading of ZnPc into mesoporous silica shell on NaYF4@silica nanoparticles by physical encapsulation and use them for PDT. (b) A schematic drawing showing Ce6 physically adsorbed on the surface of PEGylated UCNPs via hydrophobic interactions to form UCNP-Ce6 complex. c) Covalent conjugation of photosensitizer RB and target molecule FA onto amine functionalized UCNPs. Copyright 2009 Wiley,[46] 2012 Elsevier[48] and 2012 American Chemical Society.[49]

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 Figure 2 

In vitro targeted PDT using UCNPs. (a) Confocal UCL images showing specific targeting of FR positive JAR carcinoma cells by UCNP-RB-FA. (b) Relative viabilities of JAR cells (left, FR+) and NIH 3T3 fibroblasts (right, FR-) treated with UCNP-RB-FA at different concentrations with (solid) or without (open) 980-nm laser exposure. Excellet FR+ cancer cell targeting effect was evidenced by both confocal UCL images and selective PDT killing effects. Copyright 2012 American Chemical Society.[49]

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2.3 Covalent conjugation

When physically entrapped inside the silica network or the hydrophobic layer, those PS molecules may be prematurely released from the carrier which can lead to a reduced efficiency of treatment. Covalent coupling of PS molecules inside or at the surface of nanoparticles may be able to overcome these drawbacks. Zhang et al.[49] described a covalent bonding strategy to link a PS molecule, Rose Bengal (RB), onto UCNPs (Figure 2), by conjugating carboxyl groups on hyaluronic acid (HA) modified RB to amino groups on the surface of UCNPs via amide bonds. They found the covalent bonding between UCNPs and RB molecules significantly improved the PS loading capacity and the energy transfer efficiency from nanoparticles to PS molecules. In another work, Zhou et al.[50] used O-carboxymethylated chitosan to modify UCNPs. Chemical covalent linkage was employed to conjugate targeting peptide c(RGDyK) and photosensitizing pyropheophorbide a (Ppa) to UCNPs. They showed that covalently linked Ppa molecules not only were stable but also retained their spectroscopic and functional properties for targeted NIR-induced PDT. (Figure 1c)

In a very recent work, Hyeon and co-workers [51] combined both physical adsorption and chemical conjugation methods to attach PS molecules onto UCNPs. Water-dispersible and amine-functionalized UCNPs encapsulated by PEGylated phospholipids were synthesized. Owing to the hydrophobic property and planar structure of chlorin, Ce6 could be readily entrapped in hydrophobic phospholipid layers surrounding UCNPs. On the other hand, the reaction between the amine-functionalized UCNPs and the carboxy group of Ce6 was employed to form an amide bond. The amount of Ce6 loading calculated from the UV-vis absorption spectra was found to be > 10 3 Ce6 molecules per particle.

3. In vitro PDT using UCNPs

In vitro PDT using UCNP-PS nanocomplexes was demonstrated in several early cell experiments.[37, 42, 43, 52] In a study by Zhang and co-workers,[43] mesoporous silica coated NaYF4(Yb:Er) UCNPs core-shell nanoparticles were loaded with ZnPc. Upon irradiation at 980 nm, visible light emitted from UCNPs could activate ZnPc molecules in the silica shell via resonance energy transfer, showing a strong photodynamic cell destruction effect on MB49 cells. DNA ladder fragments were found after PDT treatment of MB49 cells, together with cytochrome c release from the mitochondria into the cytosol. These data showed that DNA bases and mitochondria were attacked by reactive singlet oxygen molecules, which were generated during NIR laser irradiation. A laser power at 0.5 W and an irradiation time of 5 min were needed to induce significant cell death in this work. In another report, Shan et al.[44] increased the loading ratio of photosensitizers molecules on UCNPs to be about 10 wt. % by encapsulating 100 nm UCNPs and meso-tetraphenyl porphine photosensitizers in poly(ethylene glycol-block-(DL)lactic acid). The composite nanoparticles were shown to exhibit efficient HeLa cervical cancer cell-killing activity upon 980-nm NIR excitation at 134 W/cm2 for 45 min, which, however, was an extremely high optical dosage.

UCNP for targeted NIR photodynamic therapy of cancer cells has also been reported by several groups. Liu et al.[49] used folic acid (FA) to functionalized UCNP-RB nanoconjugates. A covalent bonding strategy was used to construct a highly efficient NIR photosensitizing nanoplatform for simultaneous PDT and imaging. Specific targeting of folate receptor (FR) positive JAR carcinoma cells was evidenced by UCL cell imaging. After NIR laser irradiation at 1.5 W/cm2 for 10 min, cell viabilities decreased significantly for the JAR carcinoma cells but were not obviously changed for FR negative noncancerous NIH 3T3 cells (Figure 2). Zhou et al.[50] used a photosensitizer pyropheophorbide a (Ppa) and RGD peptide c(RGDyK) co-modified chitosan-wrapped NaYF4:Yb/Er UCNPs for targeted PDT. Selective PDT killing of integrin αvβ3 positive U87-MG tumor cells was demonstrated by using those UCNP-Ppa-RGD conjugates and 980-nm NIR laser irradiation at 300 J/cm2.

The success of using UCNP-PS nanocomplexes for in vitro PDT cancer cell killing under NIR light thus encourages further investigations of this approach for in vivo cancer treatment, which will be discussed in the following section.

4. In vivo PDT using UCNPs

The first in vivo UCNP-based PDT study in animal experiments was demonstrated by our team (Figure 3).[48] We non-covalently incorporated Ce6 onto PEGylated amphiphilic polymer-coated UCNPs. The obtained UCNP-Ce6 complex could enter cancer cells and induce 4T1 cell death after being exposed to the 980-nm light at 0.5 W/cm2 for 30 min. For animal experiments, we directly injected UCNP-Ce6 (20 mg/ml UCNP, ~1.5 mg/ml Ce6) into 4T1 murine breast cancer tumors grown on BABL/C mice. After 980-nm NIR laser excitation for 30 min (with 1-min interval for each minute to avoid heating) at 0.5 w/cm 2, excellent tumor regression effect was observed for UCNP-Ce6 injected tumors, while neither UCNP-Ce6 injection by itself nor NIR-laser irradiation alone was able to delay the tumor growth (Figure 3a). The survival time of mice after UCNP-Ce6 injection and PDT treatment was dramatically pro-longed compared to the control mice as well (Figure 3b). Moreover, we found that UCNPs injected into tumors after PDT treatment could be gradually cleared out from mouse organs after 2 months as determined by ex vivo inductively coupled plasma-atomic emission spectrometry (ICP-AES) assay (Figure 3c), without obviously rendering noticeable toxicity to the treated animals. At last, we also compared the tissue penetration abilities between UCNP-based PDT induced by 980-nm NIR light and traditional PDT using visible light at 660 nm, by blocking irradiated subjects using pork tissues with different thicknesses. Although direct exposure of a Ce6 solution to the 660-nm light generated much more singlet oxygen compared to UCNP-Ce6 sample under the 980-nm excitation (the same Ce6 concentration, the same optical power density), 1O2 formation of the free Ce6 sample was dramatically reduced if the 660-nm light was blocked by a 3-mm thick pork tissue and completely eliminated if blocked by a 8-mm thick tissue. In contrast, the 1O2 production remained a decent level for UCNP-Ce6 even when the 980-nm light was blocked by a 8-mm thick tissue. In vitro and in vivo experiments further confirmed that compared to the traditional PDT relying on visible light excitation, the NIR light triggered PDT of cancer using UCNP-Ce6 nanocomplex offers terrifically improved tissue penetration depth and could be preferable for the treatment of large or internal tumors.

In another work, Cui et al.[53] developed amphiphilic chitosan modified UCNPs loaded with ZnPc for UCNP-based PDT in vivo. Hydrophobic OA-capped UCNPs were modified with biodegradable N -succinyl-N'-octyl chitosan (SOC) to improve biocompatibility of UCNPs. ZnPc was loaded into SOC-UCNPs with high drug loading capacity through hydrophobic interactions with the SOC layer. Via intra-tumoral injection, a prominent anti-tumor effect of the ZnPc-UCNPs to S180 tumors on mice was observed after 980-nm NIR laser irradiation at 0.4 W/cm2 for 15 min. Their results are consistent with our work [48] and offer another example of in vivo UCNP-based PDT treatment in animal studies.

 Figure 3 

In vivo PDT treatment of tumor-bearing mice by UCNP-Ce6. (a) The growth of 4T1 tumors on different groups of mice after various treatments indicated. (b) The survival curves of mice in 60 days after various treatments indicated. (c) Biodistribution of Yttrium in various organs of UCNP-Ce6 injected mice after PDT treatment. ICP-AES was employed to quantitatively determine the Y 3+ levels. Our work demonstrated NIR light-induced in vivo PDT treatment of s.c. tumor in animals, and UCNPs would be cleared out from the mouse body after 2 months. Copyright 2012 Elsevier.[48]

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 Figure 4 

In vivo imaging-guided PDT. (a) UCL images of nude mice bearing tumors after intravenous injection of UCNP-Ce6. (b) T1-weighted MR images of a tumor-bearing nude mouse before and after 1.5 h intravenous injection of UCNP-Ce6. (c) Growth of tumors after various treatments indicated for efficient imaging guided PDT therapy. Copyright 2012 Wiley-VCH.[51]

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Very recently, Hyeon and co-workers [51] reported in vivo PDT effect through the systemic administration of UCNP-Ce6 followed by the 980-nm irradiation (Figure 4). NaGdY4-based UCNPs after PEGylation were loaded with Ce6 molecules by both physical adsorption and chemical conjugation, yielding a UCNP-Ce6 complex with > 103 Ce6 molecules per particle. The blood circulation half-life of UCNP-Ce6 was determined to be 21.6 min in BABL/C mice after intravenous injection. Nude mice bearing U87MG tumors were injected with UCNP-Ce6 through the tail vein (0.1 mg of rare earth per mouse). Obvious tumor accumulation of UCNP-Ce6 nanoparticles was revealed by dual-modal upconversion luminescence imaging and T1-weighted MR imaging (Figure 4a&b), and could likely be attributed to the enhanced permeability and retention (EPR) effect of cancerous tumors. Under the 980 nm irradiation, tumor growth of UCNP-Ce6 injected mice was significantly inhibited compared with other control groups (Figure 4c). These results clearly indicate the great potential of using UCNPs for multi-modal imaging guided PDT. Remarkably, this study is the first report to demonstrate UCNP-based in vivo PDT through the systemic administration of UCNP-PS complexes.

In another latest report, Zhang and coauthors demonstrated in vivo tumor-targeted PDT using UCNPs.[6] NaYF4(Yb:Er) UCNPs were coated with mesoporous silica and co-loaded with ZnPc and merocya-nine 540 (MC540), which were two widely used PS molecules with different excitation wavelengths (Figure 5). Two main peaks, green (~540 nm) and red (~660 nm) emissions of UCNPs excited by 980-nm laser matched well with the absorption of two types of PS molecules, for fully utilization of upconverted energy to maximize the PDT efficiency. ZnPc/MC540 coloaded UCNPs resulted in the high rate to generate 1O2 in the solution as well as in the live cells under the NIR light treatment. Then, they carefully assessed the therapeutic efficacy of those ZnPc/MC540 coloaded UCNP-PS complexes as an in vivo PDT agent in three sets of mouse experiments. In the first step, they injected B16-F0 melanoma cells labeled with ZnPc/MC540 coloaded UCNPs under the skin of C57BL/6 mice and then irradiated the injected site with a 980-nm laser (415 mW/cm2, 2 h), observing sounded PDT efficacy. After that, they further confirmed the PDT efficacy by intratumorally injecting ZnPc/MC540 coloaded UCNPs into C57BL/6 mice bearing melanoma tumors. Great tumor regression effect was also noticed after the injected tumors were exposed to the NIR laser. At last, they further examined the targeted PDT efficacy using PEGylated UCNPs conjugated with folic acid. Mice bearing B16-F0 melanoma tumors were intravenously injected with ZnPc/MC540 coloaded FA-PEG-UCNPs and then irradiated with a 980-nm laser at 4 h post injection. A significant reduction in tumor growth of the treatment group was observed compared to control mice treated with PBS (Figure 5). This is the first time that actively tumor-targeted PDT using UCNPs is demonstrated in vivo.

As discussed above, UCNP-based PDT has been successfully demonstrated in vivo, showing encouraging therapeutic effect upon either local injection or systemic administration of UCNP-PS nanocomplexes. Further efforts in this field may enable a new photodynamic therapeutic approach with greatly improved tissue penetration, suitable for treatment of relatively large or internal tumors.

 Figure 5 

Targeted in vivo PDT of a subcutaneous tumor model injected with FA-PEG-UCNPs. (a) The fluorescence emission spectrum of UCNPs under 980-nm NIR laser excitation and the absorption spectra of ZnPc and MC540 photosensitizers. (b) A schematic drawing showing mesoporous-silica-coated UCNPs coloaded with ZnPc and MC540 for PDT. (c) A schematic diagram showing UCNP-based targeted PDT in a mouse model of melanoma. Those ZnPc/MC540 co-loaded UCNPs were functionalized with PEG and conjugated with FA for in vivo tumor targeting. (d) Change in tumor size as a function of time after various treatments indicated. Intravenously injected FA-PEG-UCNPs co-loaded with ZnPc/MC540 was able to obviously delay the tumor growth after the 980-nm NIR laser exposure. Copyright 2012 Nature Publishing Group.[6]

Theranostics Image (Click on the image to enlarge.)

5. NIR triggered drug release and gene delivery using UCNPs

In addition to the application of UCNPs in PDT, they could also be used as an energy donor to trigger photochemistry that usually happens under UV or visible light by NIR light irradiation.[5, 54-57] Photo responsive drug release systems have received significant interests in recent years, owing to their great potential in remotely controlled release of therapeutics at the site of interest (e.g. tumors).[58] However, the majority of photochemistry that employed in photo responsive drug release systems requires high-energy UV excitation, which not only induces substantial phototoxicity, but also exhibits short penetration depth in biological systems. A specific class of UCNPs, usually containing Yb and Tm, are able to emit UV light under 980-nm NIR excitation, and could have great promise in the design of NIR-light responsive drug delivery systems.

In 2010, Carling et al.[55] for the first time demonstrated the use of NIR exciting & UV emitting (NIR-to-UV) UCNPs (NaYF4:Yb Tm) for NIR laser triggered molecular releasing. A caged compound, 3´, 5´-di(carboxymethoxy)benzoin, which was usually photo activated by UV light, was conjugated to NIR-to-UV UCNPs. Under NIR laser irradiation, UV light generated by UCNPs could trigger the un-caging process and result in the release of organic molecules, a carboxylic acid in this case, from UCNPs. In a later work, Yan et al. synthesized a UV-light responsive amphiliphic block co-polymer to co-encapsulate hydrophobic NIR-to-UV UCNPs by micelle formation.[56] When exposed to the NIR laser, UCNPs could emit UV light, which then could cleave the photo-labile hydrophobic o-nitrobenzyl groups in this co-polymer, thus resulting in the dissociation of micelles and release of co-loaded hydrophobic molecular payloads. Although these two systems are just proof-of-concept studies, the strategy presented in their reports is expected to be adopted for the design of NIR light controllable drug release platforms based on UCNPs.

Recently, Zhang and co-workers[54] reported the use of NIR-to-UV UCNPs for photo-controllable gene expression (Figure 6). Plasmid DNA encoding green fluorescence protein (GFP) and small interfering RNA (siRNA) target GFP mRNA were both caged with 4,5-dimethoxy-2-nitroacetophenone DMNPE to block their respective functions. After NIR light treatment, they were uncaged by the energy transferred from UCNPs, inducing controlled gene expression and specific gene silencing, respectively. The NIR-to-UV UCNPs overcome the drawback of current photo-responsive systems in which UV light is needed to activate DMNPE caged nucleic acids. Besides, the upconverted UV produced by the irradiated UCNPs was also found to be relatively safe for the cells, under the applied nanoparticle dosage and duration of NIR laser irradiation. This system was then further used to activate photocaged nucleic acids in animal models. Cells transfected with UCNPs containing photocaged GFP plasmid were loaded into a polydimethylsiloxane (PDMS) device, which was transplanted into mice. Efficient GFP activation was observed even at the deep tissues. Those results prove that this technique has enormous potential in a number of fields including gene therapy for controlled and specific gene delivery/knockdown, developmental biology for site-specific gene knockdown, and patterning of biomolecules using safe NIR light.

Bioluminescence imaging is a technology that allows for the noninvasive study of ongoing biological processes in small laboratory animals. Xing and co-workers [5] reported NIR light controlled uncaging of d-luciferin and bioluminescence imaging in vivo using NIR-to-UV UCNP probes. The core-shell NIR-to-UV UCNPs were coated with thiolated silane molecules and subsequently coupled to d-luciferin that was caged with a 1-(2-nitrophenyl)ethyl group. UV light emitted from UCNPs under NIR irradiation could activate caged d-luciferin to release d-luciferin molecules which was an active substrate of luciferase used in bioluminescence imaging. Cell viability assays showed no obvious cytotoxicity for C6 glioma cells treated with the d-luciferin/UCNP conjugate after two hours of irradiation with NIR light. In marked contrast, UV irradiation resulted in significant cellular damage after a short exposure time. Importantly, strong bioluminescence signals were detected in the mouse injected d-luciferin/UCNP conjugate after NIR-light induced photo uncaging. While under UV irradiation, no notable bioluminescence was detected in the mouse owing to the poor tissue penetration of UV light (Figure 7).

It is foreseeable that UCNPs might find future application as nanotransducers for highly controllable NIR photo-triggered activation of caged cancer theranostics, providing an effective route to develop spatially and temporally controlled drug delivery and imaging systems.

 Figure 6 

Schematic illustration of NIR triggered gene release using NIR-UV UCNPs. (a) Plasmid DNA or siRNA are caged with DMNPE and then uncaged by upconverted UV light emitted from NIR-to-UV UCNPs. Inset shows the penetration depth of UV and NIR light in the skin. (b) Photoactivation and patterned activation of caged GFP nucleic acids in cells. Copyright 2012 Highwire press PNAS.[54]

Theranostics Image (Click on the image to enlarge.)
 Figure 7 

Experimental design for NIR activated bioluminescence imaging. Caged luciferin conjugated on NIR-to-UV UCNPs was activated after 980-nm laser irradiation, releasing un-caged luciferin as an active substrate in bioluminescence imaging. Copyright 2012 Wiley-VCH.[5]

Theranostics Image (Click on the image to enlarge.)

6. Other therapeutic applications and imaging-guided cancer therapies using upconversion nanoparticles and their nanocomposites

Recently, UCNPs combined with other functionalities have also been explored for multifunctional biomedical imaging and drug delivery.[47, 59-69] In our study, we showed that UCNPs could be loaded with chemotherapy drug molecules for imaging and therapy simultaneously.[47] Doxorubicin (DOX) was loaded on PEGylated UCNPs by physical adsorption and could be released under slightly acidic pH. Utilizing a folate targeting model, targeted cancer cell imaging and drug delivery was achieved. In another recent work, Lin and coauthors[64] reported a drug delivery system based on DOX-conjugated NaYF4:Yb3+/Tm3+ UCNPs, which was also able to release DOX in mildly acidic environments. The LRET between UCNPs and DOX could be utilized as the optical indicator to monitor the release of DOX inside cells.

In our another work, we encapsulate UCNPs together with iron oxide nanoparticles(IONPs), fluorescent dye and/or anti-cancer drug molecules by using an amphiphilic block copolymer, poly(styrene-block-allyl alcohol) (PS16-b -PAA10), via a microemulsion method, to obtain a multi-functional nanocomposite system(Figure 8a).[65] Triple-modal upconversion luminescence (UCL )/down-conversion fluorescence (FL)/magnetic resonance (MR) imaging was demonstrated in vitro and in vivo, and also applied for in vivo cancer cell tracking in mice. In addition, after loading anti-cancer drug, DOX, into the complex, a novel imaging-guided and magnetic targeted drug delivery system was fabricated for potential multimodal biomedical imaging and cancer therapy.

 Figure 8 

Development of UCNP-based nanocomposites for multifunctional biomedical imaging and therapy. (a) A schematic illustration for UCNP-IONP@Polymer nanocomposite synthesis and applications. (b) A schematic illustration showing UCNP@IONP@Au MFNP synthesis and functionalization. Copyright 2011 Elsevier[65] and 2011 Wiley-VCH.[62]

Theranostics Image (Click on the image to enlarge.)

Different from PDT, photothermal therapy is another type of photo therapies that utilizes NIR absorbing agents to generate heat from absorbed light to 'cook' cancer by high temperature. In our group, we developed a novel class of multifunctional nanoparticles (MFNPs) based on UCNPs with combined optical and magnetic properties useful in multimodality imaging and therapy.[62, 63] The MFNPs were prepared by a layer-by-layer (LBL) self-assembly strategy. Firstly, ultra-small IONPs were adsorbed on the surface of a UCNP by electrostatic attraction. A thin gold shell was formed by seed-induced reduction growth on the UCNP-IONP complex, yielding UCNP@IONP@Au MFNPs which were then coated by lipoic acid modified PEG to impart stability in physiological solutions. The obtained PEGylated MFNPs were successfully used for in vivo dual-modal imaging guided & magnetically targeted PTT. Owing to the strong NIR optical absorption of those Au shelled MFNPs and the highly efficient magnetic tumor targeting, tumors on mice after MFNP-injection and magnetic tumor-targeting were completely eliminated upon exposure to the 808-nm NIR laser (Figure 8b).

Thus, the rapid development in controllable nanomaterials synthesis as well as finely tuned surface engineering could allow researchers to design and fabricate various UCNP-based nanocomposites with highly integrated functionalities, useful for multimodal imaging and imaging-guided cancer therapies.

7. Conclusions and perspective

The past decade has witnessed an explosion of interest in the use of UCNPs as novel theranostic probes in biomedicine. As summarized in this review article, applications of UCNPs for photodynamic therapy, NIR triggered drug and gene delivery, as well as several other UCNP-based cancer therapeutic approaches have been demonstrated. However, in the near future, there may still be many obstacles to be overcome.

Firstly, brighter UCNPs need to be synthesized so that stronger energy could be transferred to PS for stronger photodynamic effect. The quantum yield of UCL emission of UCNPs is mostly less than 1%, thus limiting the use of these nano-probes in optical imaging and photodynamic therapy.[70] Tremendous efforts are still needed to frabricate new types of UCNPs with high quantum yield, by designing special UCNP structures (e.g. core-shell structures) [71] and choosing better host matrix materials (e.g. NaLuY4).[72]

Currently, most of UCNPs are excited by the 980-nm light. The imaging penetration depth has been reported to be as thick as 1.6 cm by Li and co-workers[26], and 3.2 cm by Han and co-workers[11]. Although the 980-nm excitation does offer excellent tissue penetration, water also has an absorption peak at around 980 nm, resulting in unnecessary tissue heating during PDT treatment. Development of UCNPs that can be effectively excited by other NIR wavelengths (e.g. 915 nm as shown by Zhan et al.)[73] may help to solve this problem. Using excitation light at 915 nm with much lower water absorption only not could avoid skin heating, but may also allow deeper tissue penetration in UCNP-based imaging and PDT treatment.

Better surface functionalization and bioconjugation strategies should also be developed. Most of currently used UCNPs would accumulate in reticuloendothelial systems (RES) such as liver, spleen after systemic administration.[74-76] There is only one example to date to realize active tumor targeted PDT in animals using the folate system,[6] which unfortunately has limited clinical value. There is considerable scope for further development of UCNPs with better surface functionalization to enhance their tumor targeting as well as to reduce their RES retention.

Lastly, before using these UCNPs for clinical therapeutic purposes, overall understanding of nanotoxicology of UCNPs including nanoparticle distribution, excretion, metabolism, pharmacokinetics and pharmacodynamics in animal models should be continually studied in the future. The toxicity of UCNPs is closely associated to their surface chemistry, and biocompatible surface coatings would significantly reduce their cytotoxicity. Current studies have shown that functionalized NaYF4-based UCNPs with biocompatible coatings appear to be not obviously toxic to cells at certain concentration ranges to treated animals over several months.[74-76] However, further in-depth exploration of the physicochemical and physiological processes of UCNPs in vivo is essential such as how this class of nanomaterials would do to animals over an even longer time course, how they interact with immune systems, and whether they may interfere the reproductive system and affect the next generation.

Despite the above challenges, UCNPs with unique optical properties indeed have significant potential in biomedicine. For the imaging, little auto-fluorescence during UCL imaging makes them excellent probes especially for in vivo imaging with extremely high sensitivity (e.g. down to single cell detection limit in animals)[23, 25, 27]. For the NIR-excited PDT or other NIR-triggered theranostics using UCNPs, NIR light offers unmatched advantages compared with UV or visible light used in current methodologies. Better design of materials to achieve desired performance and biological behaviors, as well as more in-depth investigation in biomedicine, may further push forward the theranostic applications of UCNPs.

Acknowledgements

This work was partially supported by the National Basic Research Programs of China (973 Program) (2012CB932600, 2011CB911002), the National Natural Science Foundation of China (51002100), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Competing Interests

The authors have declared that no competing interest exists.

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Author contact

Corresponding address Corresponding author: E-mail: zliuedu.cn.


Received 2012-9-24
Accepted 2012-10-23
Published 2013-3-25