Theranostics 2012; 2(2):198-206. doi:10.7150/thno.3477

Research Paper

Measurement of MMP Activity in Synovial Fluid in Cases of Osteoarthritis and Acute Inflammatory Conditions of the Knee Joints Using a Fluorogenic Peptide Probe-Immobilized Diagnostic Kit

Ju Hee Ryu1,2, #, Aeju Lee1, 3,#, Myung Sook Huh1, Junuk Chu1, Kwangmeyung Kim1, Byung-Soo Kim2, Kuiwon Choi1, Ick Chan Kwon1, Jong Woong Park4✉, Inchan Youn1 ✉

1. Biomedical Research Center, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul, South Korea;
2. School of Chemical and Biological Engineering, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-744, South Korea;
3. Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, South Korea;
4. Department of Orthopaedic Surgery, College of Medicine, Korea University, Seoul, South Korea.
#These authors contributed equally to this paper.

Abstract

Purpose: A fluorogenic peptide probe-immobilized diagnostic kit was used to analyze MMP activity in the synovial fluids (SFs) from patients with osteoarthritis (OA) and acute inflammatory conditions of the knee joint.

Methods: The MMP diagnostic kit containing a polymer-conjugated MMP probe immobilized on a 96-well plate was utilized for high-throughput screening of MMP activity in SFs from OA patients (n = 33) and patients with acute inflammatory conditions of the knee joint (n = 5).

Results: Compared to SF from OA patients, SF from patients with acute inflammatory conditions of the knee joint presented stronger NIR fluorescent signals. In gelatin zymography, most samples from patients with acute inflammatory conditions of the knee joint also displayed 92 kDa (pro-form) MMP-9 and faint 84 kDa (active form) MMP-9, while SF from OA patients did not display detectable MMP-9 activity .

Conclusion: The presence of a strong fluorescence signal from the MMP diagnostic kit corresponded well with patients with acute inflammatory conditions of the knee joint. The results suggest that our MMP diagnostic kit can be useful in differentiation between early stages of OA and acute inflammatory conditions of the knee joint.

Keywords: Matrix metalloproteinases, Osteoarthritis, Synovial fluid, Diagnostic kit, Acute inflammatory conditions of the knee joint

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How to cite this article:
Ryu JH, Lee A, Huh MS, Chu J, Kim K, Kim BS, Choi K, Kwon IC, Park JW, Youn I. Measurement of MMP Activity in Synovial Fluid in Cases of Osteoarthritis and Acute Inflammatory Conditions of the Knee Joints Using a Fluorogenic Peptide Probe-Immobilized Diagnostic Kit. Theranostics 2012; 2(2):198-206. doi:10.7150/thno.3477. Available from http://www.thno.org/v02p0198.htm