Theranostics 2011; 1:230-239. doi:10.7150/thno/v01p0230

Research Paper

Photosensitizer-Conjugated Human Serum Albumin Nanoparticles for Effective Photodynamic Therapy

Hayoung Jeong1,2 *, MyungSook Huh1 *, So Jin Lee1, Heebeom Koo1, Ick Chan Kwon1, Seo Young Jeong2 ✉, Kwangmeyung Kim1 ✉

1. Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-Dong, Sungbook-Gu, Seoul 136-791, Korea.
2. Department of Life and Nanopharmaceutical Science, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea.
* These authors contributed equally to this paper.

Abstract

Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. The focus of this study was the development of tumor-targeting albumin nanoparticles containing photosensitizers for efficient PDT. To produce tumor-targeting albumin nanoparticles, the hydrophobic photosensitizer, chlorin e6 (Ce6), was chemically conjugated to human serum albumin (HSA). The conjugates formed self-assembled nanoparticle structures with an average diameter of 88 nm under aqueous conditions. As expected, the Ce6-conjugated HSA nanoparticles (Ce6-HSA-NPs) were nontoxic in their native state, but upon illumination with the appropriate wavelength of light, they produced singlet oxygen and damaged target tumor cells in a cell culture system. Importantly, when the nanoparticles were injected through the tail vein into tumor-bearing HT-29 mice, Ce6-HSA-NPs compared with free Ce6 revealed enhanced tumor-specific biodistribution and successful therapeutic results following laser irradiation. These results suggest that highly tumor-specific albumin nanoparticles have the potential to serve not only as efficient therapeutic agents, but also as photodynamic imaging (PDI) reagents in cancer treatment.

Keywords: Photodynamic therapy, Human Serum Albumin Nanoparticles

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How to cite this article:
Jeong H, Huh M, Lee SJ, Koo H, Kwon IC, Jeong SY, Kim K. Photosensitizer-Conjugated Human Serum Albumin Nanoparticles for Effective Photodynamic Therapy. Theranostics 2011; 1:230-239. doi:10.7150/thno/v01p0230. Available from http://www.thno.org/v01p0230.htm