Theranostics 2020; 10(13):6061-6071. doi:10.7150/thno.42114 This issue Cite

Research Paper

Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking

Cai Zhang, Zhiwen Xu, Huixia Di, Erzao Zeng, Ying Jiang, Dingbin Liu

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, China

Citation:
Zhang C, Xu Z, Di H, Zeng E, Jiang Y, Liu D. Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics 2020; 10(13):6061-6071. doi:10.7150/thno.42114. https://www.thno.org/v10p6061.htm
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Abstract

Graphic abstract

In vivo tracking of dendritic cell (DC) migration to the lymphatic system is essential for evaluating the outcome of DC-based immunotherapies. Novel multimodal imaging strategies with high analytical performance are urgently needed to supply complementary information about the migration and colonization of DCs. In this study, we designed a bimodal imaging agent, namely Au@Prussian blue-Gd@ovalbumin nanoparticles (APG@OVA NPs), for activating DCs and real-time tracking of DC migration process by magnetic resonance imaging (MRI). Moreover, the distribution of the colonized DCs in the lymphatic system was profiled at the single-cell levels based on surface-enhanced Raman scattering (SERS) technique.

Methods: In this strategy, PBs as cyanide (CN)-bridged coordination blocks were assembled onto the gold nanoparticles core to provide SERS signal in the Raman-silent region (1800 and 2800 cm-1), which could avoid background signal interference. The doping Gd3+ located in the lattice of PB enables the MRI ability with high relaxivity of the probe. Ovalbumin, an egg allergen, was used as an antigen to activate DCs due to its immunological properties. The prepared APG@OVA NP agents were used to activate DCs with high efficacy and to track their migration and distribution in vivo through SERS/MR bimodal imaging.

Results: The APG@OVA NP agents could not only enable DC activating and labeling, but also achieve real-time monitoring of DC migration in vivo and accurate profiling of DC distribution in the lymphatic system. MR imaging indicated the time-dependent migration of the APG@OVA NP-labeled DCs from the footpad to the sentinel lymph node. The background-free Raman mapping of the lymph node tissue slice demonstrated that the activated DCs have successfully colonized to the sentinel lymph node.

Conclusion: Concerning the high activating efficacy, dual complementary imaging readouts, and low biological toxicity, the APG@OVA NPs act as high-performance tracking agents for DC-based immunotherapies.

Keywords: dendritic cell, Au@prussian, bimodal agents


Citation styles

APA
Zhang, C., Xu, Z., Di, H., Zeng, E., Jiang, Y., Liu, D. (2020). Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics, 10(13), 6061-6071. https://doi.org/10.7150/thno.42114.

ACS
Zhang, C.; Xu, Z.; Di, H.; Zeng, E.; Jiang, Y.; Liu, D. Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics 2020, 10 (13), 6061-6071. DOI: 10.7150/thno.42114.

NLM
Zhang C, Xu Z, Di H, Zeng E, Jiang Y, Liu D. Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics 2020; 10(13):6061-6071. doi:10.7150/thno.42114. https://www.thno.org/v10p6061.htm

CSE
Zhang C, Xu Z, Di H, Zeng E, Jiang Y, Liu D. 2020. Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics. 10(13):6061-6071.

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