Theranostics 2013; 3(11):916-926. doi:10.7150/thno.6943 This issue Cite

Research Paper

Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment

Dorota A. Kedziorek1, Meiyappan Solaiyappan1, Piotr Walczak1,2, Tina Ehtiati3, Yingli Fu1, Jeff W.M. Bulte1,2, Steven M. Shea1,3, Alexander Brost4, Frank K. Wacker1,5, Dara L. Kraitchman1✉

1. Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States.
2. Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States.
3. Center for Applied Medical Imaging, Corporate Technology, Siemens Corporation, Baltimore, Maryland, United States.
4. Pattern Recognition Lab, University of Erlangen, Erlangen, Germany.
5. Department of Radiology, Hannover Medical School, Hannover, Germany.

Citation:
Kedziorek DA, Solaiyappan M, Walczak P, Ehtiati T, Fu Y, Bulte JWM, Shea SM, Brost A, Wacker FK, Kraitchman DL. Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment. Theranostics 2013; 3(11):916-926. doi:10.7150/thno.6943. https://www.thno.org/v03p0916.htm
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Abstract

Poor cell survival and difficulties with visualization of cell delivery are major problems with current cell transplantation methods. To protect cells from early destruction, microencapsulation methods have been developed. The addition of a contrast agent to the microcapsule also could enable tracking by MR, ultrasound, and X-ray imaging. However, determining the cell viability within the microcapsule still remains an issue. Reporter gene imaging provides a way to determine cell viability, but delivery of the reporter probe by systemic injection may be hindered in ischemic diseases.

In the present study, mesenchymal stem cells (MSCs) were transfected with triple fusion reporter gene containing red fluorescent protein, truncated thymidine kinase (SPECT/PET reporter) and firefly luciferase (bioluminescence reporter). Transfected cells were microencapsulated in either unlabeled or perfluorooctylbromide (PFOB) impregnated alginate. The addition of PFOB provided radiopacity to enable visualization of the microcapsules by X-ray imaging. Before intramuscular transplantation in rabbit thigh muscle, the microcapsules were incubated with D-luciferin, and bioluminescence imaging (BLI) was performed immediately. Twenty-four and forty-eight hours post transplantation, c-arm CT was used to target the luciferin to the X-ray-visible microcapsules for BLI cell viability assessment, rather than systemic reporter probe injections. Not only was the bioluminescent signal emission from the PFOB-encapsulated MSCs confirmed as compared to non-encapsulated, naked MSCs, but over 90% of injection sites of PFOB-encapsulated MSCs were visible on c-arm CT. The latter aided in successful targeting of the reporter probe to injection sites using conventional X-ray imaging to determine cell viability at 1-2 days post transplantation. Blind luciferin injections to the approximate location of unlabeled microcapsules resulted in successful BLI signal detection in only 18% of injections. In conclusion, reporter gene probes can be more precisely targeted using c-arm CT for in vivo transplant viability assessment, thereby avoiding large and costly systemic injections of a reporter probe.

Keywords: mesenchymal stem cells, reporter gene, microencapsulation, bioluminescence, c-arm CT, probe targeting.


Citation styles

APA
Kedziorek, D.A., Solaiyappan, M., Walczak, P., Ehtiati, T., Fu, Y., Bulte, J.W.M., Shea, S.M., Brost, A., Wacker, F.K., Kraitchman, D.L. (2013). Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment. Theranostics, 3(11), 916-926. https://doi.org/10.7150/thno.6943.

ACS
Kedziorek, D.A.; Solaiyappan, M.; Walczak, P.; Ehtiati, T.; Fu, Y.; Bulte, J.W.M.; Shea, S.M.; Brost, A.; Wacker, F.K.; Kraitchman, D.L. Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment. Theranostics 2013, 3 (11), 916-926. DOI: 10.7150/thno.6943.

NLM
Kedziorek DA, Solaiyappan M, Walczak P, Ehtiati T, Fu Y, Bulte JWM, Shea SM, Brost A, Wacker FK, Kraitchman DL. Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment. Theranostics 2013; 3(11):916-926. doi:10.7150/thno.6943. https://www.thno.org/v03p0916.htm

CSE
Kedziorek DA, Solaiyappan M, Walczak P, Ehtiati T, Fu Y, Bulte JWM, Shea SM, Brost A, Wacker FK, Kraitchman DL. 2013. Using C-Arm X-Ray Imaging to Guide Local Reporter Probe Delivery for Tracking Stem Cell Engraftment. Theranostics. 3(11):916-926.

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