Theranostics 2017; 7(6):1633-1649. doi:10.7150/thno.17588 This issue Cite

Research Paper

Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy

Ling Li1, Xia Li1, Yuzhe Wu2, Linjiang Song1, Xi Yang1, Tao He1, Ning Wang1, Suleixin Yang1, Yan Zeng1, Qinjie Wu1, Zhiyong Qian1, Yuquan Wei1, Changyang Gong1✉

1. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China;
2. College of Materials, Xiamen University, Xiamen 361005, P.R. China.

Citation:
Li L, Li X, Wu Y, Song L, Yang X, He T, Wang N, Yang S, Zeng Y, Wu Q, Qian Z, Wei Y, Gong C. Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy. Theranostics 2017; 7(6):1633-1649. doi:10.7150/thno.17588. https://www.thno.org/v07p1633.htm
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Abstract

Graphic abstract

Cancer stem cell-like cells (CSCL) are responsible for tumor recurrence associated with conventional therapy (e.g. surgery, radiation, and chemotherapy). Here, we developed a novel multifunctional nucleus-targeting nanoparticle-based gene delivery system which is capable of targeting and eradicating CSCL. These nanoparticles can facilitate efficient endosomal escape and spontaneously penetrate into nucleus without additional nuclear localization signal. They also induced extremely high gene transfection efficiency (>95%) even in culture medium containing 30% serum, which significantly surpassed that of some commercial transfection reagents, such as Lipofectamine 2000 and Lipofectamine 3000 etc. Especially, when loaded with the TRAIL gene, this system mediated remarkable depletion of CSCL. Upon systemic administration, the nanoparticles accumulated in tumor sites while sparing the non-cancer tissues and significantly inhibited the growth of tumors with no evident systemic toxicity. Taken together, our results suggest that these novel multifunctional, nucleus-targeting nanoparticles are a very promising in vivo gene delivery system capable of targeting CSCL and represent a new treatment candidate for improving the survival of cancer patients.

Keywords: gene delivery, nuclear targeting, hyaluronic acid, TRAIL, cancer stem cells, colon cancer.


Citation styles

APA
Li, L., Li, X., Wu, Y., Song, L., Yang, X., He, T., Wang, N., Yang, S., Zeng, Y., Wu, Q., Qian, Z., Wei, Y., Gong, C. (2017). Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy. Theranostics, 7(6), 1633-1649. https://doi.org/10.7150/thno.17588.

ACS
Li, L.; Li, X.; Wu, Y.; Song, L.; Yang, X.; He, T.; Wang, N.; Yang, S.; Zeng, Y.; Wu, Q.; Qian, Z.; Wei, Y.; Gong, C. Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy. Theranostics 2017, 7 (6), 1633-1649. DOI: 10.7150/thno.17588.

NLM
Li L, Li X, Wu Y, Song L, Yang X, He T, Wang N, Yang S, Zeng Y, Wu Q, Qian Z, Wei Y, Gong C. Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy. Theranostics 2017; 7(6):1633-1649. doi:10.7150/thno.17588. https://www.thno.org/v07p1633.htm

CSE
Li L, Li X, Wu Y, Song L, Yang X, He T, Wang N, Yang S, Zeng Y, Wu Q, Qian Z, Wei Y, Gong C. 2017. Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy. Theranostics. 7(6):1633-1649.

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