Theranostics 2017; 7(4):935-944. doi:10.7150/thno.17544

Research Paper

An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1

Yan Li1, Ya Xiong3, Lichao Fang1, Lili Jiang1, Hui Huang1, Jun Deng1, Wenbin Liang1, 2✉, Junsong Zheng1✉

1. Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China;
2. Department of Clinical Biochemistry, Laboratory Sciences, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China;
3. Department of Dermatology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.

Abstract

The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy. The successful approach of the simultaneous detection and quantification of two bacteria by an electrochemical biosensor demonstrated here could be readily expanded for the estimation of a variety of other pathogenic bacteria, proteins, and nucleotides. Because of their high sensitivity, electrochemical biosensors may represent a new avenue for early diagnosis of diseases.

Keywords: Electrochemical biosensor, Multifunctional nanoconjugates, Simultaneous detection, Escherichia coli O157:H7, Vibrio cholerae O1.

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How to cite this article:
Li Y, Xiong Y, Fang L, Jiang L, Huang H, Deng J, Liang W, Zheng J. An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1. Theranostics 2017; 7(4):935-944. doi:10.7150/thno.17544. Available from http://www.thno.org/v07p0935.htm