Theranostics 2016; 6(11):1975-1987. doi:10.7150/thno.16547

Research Paper

Reversal of Vascular Calcification and Aneurysms in a Rat Model Using Dual Targeted Therapy with EDTA- and PGG-Loaded Nanoparticles

Nasim Nosoudi1, Aniqa Chowdhury1, Steven Siclari1, Saketh Karamched1, Vaideesh Parasaram1, Joe Parrish1, Patrick Gerard2, Narendra Vyavahare1✉

1. Department of Bioengineering, Clemson University, Clemson, SC 29634, USA
2. Department of Mathematical Sciences, Clemson University, Clemson, SC 29634, USA.

Abstract

Degeneration of elastic lamina and vascular calcification are common features of vascular pathology such as aortic aneurysms. We tested whether dual therapy with targeted nanoparticles (NPs) can remove mineral deposits (by delivery of a chelating agent, ethylene diamine tetraacetic acid (EDTA)) and restore elastic lamina (by delivery of a polyphenol, pentagalloyl glucose (PGG)) to reverse moderate aneurysm development. EDTA followed by PGG NP delivery led to reduction in macrophage recruitment, matrix metalloproteinase (MMP) activity, elastin degradation and calcification in the aorta as compared to delivery of control blank NPs. Such dual therapy restored vascular elastic lamina and improved vascular function as observed by improvement in circumferential strain. Therefore, dual targeted therapy may be an attractive option to remove mineral deposits and restore healthy arterial structures in moderately developed aneurysms.

Keywords: AAA, calcification, chelation therapy, elastin, PGG.

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How to cite this article:
Nosoudi N, Chowdhury A, Siclari S, Karamched S, Parasaram V, Parrish J, Gerard P, Vyavahare N. Reversal of Vascular Calcification and Aneurysms in a Rat Model Using Dual Targeted Therapy with EDTA- and PGG-Loaded Nanoparticles. Theranostics 2016; 6(11):1975-1987. doi:10.7150/thno.16547. Available from http://www.thno.org/v06p1975.htm