Theranostics 2015; 5(9):1021-1029. doi:10.7150/thno.12389

Research Paper

An Innovative Strategy for the Fabrication of Functional Cell Sheets Using an Electroactive Conducting Polymer

HyungJae Lee, Youngnam Cho

New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsandong-gu, Goyang, Gyeonggi-do 410-769, South Korea

Abstract

Here, we report the development of an electric field-assisted methodology for constructing 3D C2C12 cell sheets with the potential for cell surface modification. In this method, a conducting polymer, polypyrrole (Ppy), is electrodeposited via biotin doping, and then chemical conjugation of biotinylated bone morphogenetic protein 2 (BMP2) is achieved using a biotin-streptavidin cross-linker. Subsequently, C2C12 cells are cultured on BMP2-immobilized Ppy surfaces to induce interactions between cell surface receptors and bound BMP2 ligands. Following these procedures, layers of BMP2-immobilized cells can be easily detached from the Ppy surface by applying an electrical potential. This novel method results in high affinity, ligand-bound cell sheets, which exhibit homogeneous coverage with membrane-bound proteins and signal activation that occurs via maximal receptor accessibility. Using this strategy to engineer the cell surface with desirable ligands results in structures that mimic in vivo tissues; thus, the method reported here has potential applications in regenerative medicine and tissue engineering.

Keywords: cell sheet, conducting polymer, osteogenesis, bone morphogenetic protein 2, electrical stimulation

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How to cite this article:
Lee H, Cho Y. An Innovative Strategy for the Fabrication of Functional Cell Sheets Using an Electroactive Conducting Polymer. Theranostics 2015; 5(9):1021-1029. doi:10.7150/thno.12389. Available from http://www.thno.org/v05p1021.htm