Theranostics 2014; 4(3):316-335. doi:10.7150/thno.7819

Research Paper

Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)

Sudip Mukherjee1, Debabrata Chowdhury2*, Rajesh Kotcherlakota1*, Sujata Patra1, Vinothkumar B1, Manika Pal Bhadra2, Bojja Sreedhar3, Chitta Ranjan Patra1 ✉

1. Biomaterials Group, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad - 500007, AP, India;
2. Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad - 500007, AP, India;
3. Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad - 500007, AP, India.
* Contributed equally.

Abstract

In this report, we have designed a simple and efficient green chemistry approach for the synthesis of colloidal silver nanoparticles (b-AgNPs) that is formed by the reduction of silver nitrate (AgNO3) solution using Olax scandens leaf extract. The colloidal b-AgNPs, characterized by various physico-chemical techniques exhibit multifunctional biological activities (4-in-1 system). Firstly, bio-synthesized silver nanoparticles (b-AgNPs) shows enhanced antibacterial activity compared to chemically synthesize silver nanoparticles (c-AgNPs). Secondly, b-AgNPs show anti-cancer activities to different cancer cells (A549: human lung cancer cell lines, B16: mouse melanoma cell line & MCF7: human breast cancer cells) (anti-cancer). Thirdly, these nanoparticles are biocompatible to rat cardiomyoblast normal cell line (H9C2), human umbilical vein endothelial cells (HUVEC) and Chinese hamster ovary cells (CHO) which indicates the future application of b-AgNPs as drug delivery vehicle. Finally, the bio-synthesized AgNPs show bright red fluorescence inside the cells that could be utilized to detect the localization of drug molecules inside the cancer cells (a diagnostic approach). All results together demonstrate the multifunctional biological activities of bio-synthesized AgNPs (4-in-1 system) that could be applied as (i) anti-bacterial & (ii) anti-cancer agent, (iii) drug delivery vehicle, and (iv) imaging facilitator. To the best of our knowledge, there is not a single report of biosynthesized AgNPs that demonstrates the versatile applications (4-in-1 system) towards various biomedical applications. Additionally, a plausible mechanistic approach has been explored for the synthesis of b-AgNPs and its anti-bacterial as well as anti-cancer activity. We strongly believe that bio-synthesized AgNPs will open a new direction towards various biomedical applications in near future.

Keywords: Bio-synthesis, Silver nanoparticle, Green Chemistry, Olax scandens, Multifunctional activities, Antibacterial, anti-cancer.

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How to cite this article:
Mukherjee S, Chowdhury D, Kotcherlakota R, Patra S, B V, Bhadra MP, Sreedhar B, Patra CR. Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System). Theranostics 2014; 4(3):316-335. doi:10.7150/thno.7819. Available from http://www.thno.org/v04p0316.htm